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Rapeseed flower pollen bio-green synthesized silver nanoparticles: a promising antioxidant, anticancer and antiangiogenic compound

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Abstract

Based on recent researches, bio synthesized silver nanoparticles (Ag-NPs) seem to have the potential in declining angiogenesis and oxidative stress. In the current study, rapeseed flower pollen (RFP) water extract was triggered to synthesize RFP–silver nanoparticles (RFP/Ag-NPs). Moreover, antioxidant, antiangiogenesis and cytotoxicity of the RFP/Ag-NPs against MDA-MB-231, MCF7 and carcinoma cell lines and normal human skin fibroblast HDF were compared. Results indicated that RFP/Ag-NPs have a peak at 430 nm, spherical shape and an average size of 24 nm. According to the results of FTIR, rapeseed pollen capped Ag-NPs. RFP/Ag-NPs have cytotoxicity on MDA-MB-231 and MCF7 cells and decrease cancerous cell viability (IC50 = 3 µg/ml and 2 µg/ml, respectively) in a dose- and time-dependent manner. The morphological data showed that the RFP/Ag-NPs increase the percentage of apoptotic cells compared to the control group and normal cells (human skin fibroblast cells). The apoptotic morphological change was also confirmed with a flow cytometric analysis. RFP/ Ag-NPs’ antioxidant activity was evaluated by measuring their ability to scavenge ABTS and DPPH free radicals. The IC50 values were determined at 800 and 830 μg/ml for ABTS and DPPH tests, respectively. According to the results, green-synthesized RFP/Ag-NPs as a safe efficient apoptosis inducer and strong antioxidant compound have the potential to suppress breast cancer carcinogenesis by VEGF down-regulatiion and thus sensitizing them against apoptosis. However, further researches are required to clarify RFP/Ag-NPs’ cell specificity and therapeutic doses in in vivo conditions.

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References

  1. Ames BN, Shigenaga MK, Hagen TM (1993) Oxidants, antioxidants, and the degenerative diseases of aging. Proc Natl Acad Sci 90(17):7915–7922

    Article  CAS  PubMed  Google Scholar 

  2. Valko M et al (2006) Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chem Biol Interact 160(1):1–40

    Article  CAS  PubMed  Google Scholar 

  3. Sharma CK et al (2015) Green synthesis of different nanoparticles and their potential applications in different fields—a critical review. Int J Pharm Bio Sci 6(3):555–567

    CAS  Google Scholar 

  4. Iravani S (2011) Green synthesis of metal nanoparticles using plants. Green Chem 13(10):2638–2650

    Article  CAS  Google Scholar 

  5. Gejo G, Kuruvilla J, Boudenne A, Sabu T (2010) Recent advances in green composites. Key Eng Mater 425:107–166

    Article  CAS  Google Scholar 

  6. Zhang X-F et al (2016) Silver nanoparticles: synthesis, characterization, properties, applications, and therapeutic approaches. Int J Mol Sci 17(9):1534

    Article  CAS  PubMed Central  Google Scholar 

  7. Lin N, Dufresne A (2014) Nanocellulose in biomedicine: current status and future prospect. Eur Polym J 59:302–325

    Article  CAS  Google Scholar 

  8. Shah M et al (2015) Green synthesis of metallic nanoparticles via biological entities. Materials 8(11):7278–7308

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Mandal D et al (2006) The use of microorganisms for the formation of metal nanoparticles and their application. Appl Microbiol Biotechnol 69(5):485–492

    Article  CAS  PubMed  Google Scholar 

  10. Hong P et al (2015) Green synthesis and stability evaluation of ag nanoparticles using bamboo hemicellulose. BioResources 11(1):385–399

    Article  CAS  Google Scholar 

  11. Marchiol L (2012) Synthesis of metal nanoparticles in living plants. Ital J Agron 7(3):37

    Article  Google Scholar 

  12. Makarov VV, Love AJ, Sinitsyna OV, Makarova SS, Yaminsky IV, Taliansky ME, Kalinina NO (2014) “Green” nanotechnologies: synthesis of metal nanoparticles using plants. Acta Naturae (aнглoязычнaя вepcия) 6(1):35–44

    Article  CAS  Google Scholar 

  13. Kannan R, Stirk W, Van Staden J (2013) Synthesis of silver nanoparticles using the seaweed Codium capitatum PC Silva (Chlorophyceae). S Afr J Bot 86:1–4

    Article  CAS  Google Scholar 

  14. Fu D-H et al (2016) Research progress and strategies for multifunctional rapeseed: a case study of China. J Integr Agric 15(8):1673–1684

    Article  Google Scholar 

  15. Rajan R et al (2015) Plant extract synthesized silver nanoparticles: an ongoing source of novel biocompatible materials. Ind Crops Prod 70:356–373

    Article  CAS  Google Scholar 

  16. Ovais M et al (2018) Role of plant phytochemicals and microbial enzymes in biosynthesis of metallic nanoparticles. Appl Microbiol Biotechnol 102(16):6799–6814

    Article  CAS  PubMed  Google Scholar 

  17. Gasser CA et al (2014) Advanced enzymatic elimination of phenolic contaminants in wastewater: a nano approach at field scale. Appl Microbiol Biotechnol 98(7):3305–3316

    Article  CAS  PubMed  Google Scholar 

  18. Koyyati R et al (2014) Antibacterial activity of silver nanoparticles synthesized using Amaranthus viridis twig extract. Int J Res Pharm Sci 5(1):32–39

    Google Scholar 

  19. Shankar S, Rhim J-W (2015) Amino acid mediated synthesis of silver nanoparticles and preparation of antimicrobial agar/silver nanoparticles composite films. Carbohydr Polym 130:353–363

    Article  CAS  Google Scholar 

  20. Thombre R et al (2013) Synthesis of silver nanoparticles and its cytotoxic effect against THP-1 cancer cell line. Int J Pharm Bio Sci 4:184–192

    CAS  Google Scholar 

  21. Okafor F et al (2013) Green synthesis of silver nanoparticles, their characterization, application and antibacterial activity. Int J Environ Res Public Health 10(10):5221–5238

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. Namvar F et al (2016) Nanosized silver–palm pollen nanocomposite, green synthesis, characterization and antimicrobial activity. Res Chem Intermed 42(3):1571–1581

    Article  CAS  Google Scholar 

  23. Shameli K et al (2012) Investigation of antibacterial properties silver nanoparticles prepared via green method. Chem Cent J 6(1):73

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. Tippayawat P et al (2016) Green synthesis of silver nanoparticles in aloe vera plant extract prepared by a hydrothermal method and their synergistic antibacterial activity. PeerJ 4:e2589

    Article  PubMed  PubMed Central  Google Scholar 

  25. Madhanraj R, Eyini M, Balaji P (2017) Antioxidant assay of gold and silver nanoparticles from edible basidiomycetes mushroom fungi. Free Radic Antioxid 7(2):137–142

    Article  CAS  Google Scholar 

  26. Saddick S, Afifi M, Zinada OAA (2017) Effect of zinc nanoparticles on oxidative stress-related genes and antioxidant enzymes activity in the brain of Oreochromis niloticus and Tilapia zillii. Saudi J Biol Sci 24(7):1672–1678

    Article  CAS  PubMed  Google Scholar 

  27. Sulaiman GM et al (2013) Green synthesis, antimicrobial and cytotoxic effects of silver nanoparticles using Eucalyptus chapmaniana leaves extract. Asian Pac J Trop Biomed 3(1):58–63

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  28. Alon T et al (1995) Vascular endothelial growth factor acts as a survival factor for newly formed retinal vessels and has implications for retinopathy of prematurity. Nat Med 1(10):1024

    Article  CAS  PubMed  Google Scholar 

  29. Benjamin LE, Keshet E (1997) Conditional switching of vascular endothelial growth factor (VEGF) expression in tumors: induction of endothelial cell shedding and regression of hemangioblastoma-like vessels by VEGF withdrawal. Proc Natl Acad Sci 94(16):8761–8766

    Article  CAS  PubMed  Google Scholar 

  30. Gerber H-P, Dixit V, Ferrara N (1998) Vascular endothelial growth factor induces expression of the antiapoptotic proteins Bcl-2 and A1 in vascular endothelial cells. J Biol Chem 273(21):13313–13316

    Article  CAS  PubMed  Google Scholar 

  31. Biroccio A et al (2000) Bcl-2 overexpression and hypoxia synergistically act to modulate vascular endothelial growth factor expression and in vivo angiogenesis in a breast carcinoma line. FASEB J 14(5):652–660

    Article  CAS  PubMed  Google Scholar 

  32. Fernandez A et al (2001) Angiogenic potential of prostate carcinoma cells overexpressing bcl-2. J Natl Cancer Inst 93(3):208–213

    Article  CAS  PubMed  Google Scholar 

  33. Iervolino A et al (2002) Bcl-2 overexpression in human melanoma cells increases angiogenesis through VEGF mRNA stabilization and HIF-1-mediated transcriptional activity. FASEB J 16(11):1453–1455

    Article  CAS  PubMed  Google Scholar 

  34. Le Gouill S et al (2004) VEGF induces Mcl-1 up-regulation and protects multiple myeloma cells against apoptosis. Blood 104(9):2886–2892

    Article  CAS  PubMed  Google Scholar 

  35. Cuconati A et al (2003) DNA damage response and MCL-1 destruction initiate apoptosis in adenovirus-infected cells. Genes Dev 17(23):2922–2932

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  36. Willis SN et al (2005) Proapoptotic Bak is sequestered by Mcl-1 and Bcl-xL, but not Bcl-2, until displaced by BH3-only proteins. Genes Dev 19(11):1294–1305

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Acknowledgements

This work was supported by the Islamic Azad University, Mashhad, Iran, which is appreciated by the authors.

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Authors and Affiliations

  1. Department of Biology, Islamic Azad University, Mashhad Branch, Mashhad, Iran

    Sahar Hajebi, Masoud Homayouni Tabrizi, Mahboobeh Nakhaei Moghaddam, Farzaneh Shahraki & Soheyla Yadamani

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  1. Sahar Hajebi
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  2. Masoud Homayouni Tabrizi
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  3. Mahboobeh Nakhaei Moghaddam
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  4. Farzaneh Shahraki
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  5. Soheyla Yadamani
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Correspondence to Masoud Homayouni Tabrizi.

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Hajebi, S., Tabrizi, M.H., Moghaddam, M.N. et al. Rapeseed flower pollen bio-green synthesized silver nanoparticles: a promising antioxidant, anticancer and antiangiogenic compound. J Biol Inorg Chem 24, 395–404 (2019). https://doi.org/10.1007/s00775-019-01655-4

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  • DOI: https://doi.org/10.1007/s00775-019-01655-4

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