Accreditation and Quality Assurance

, Volume 23, Issue 2, pp 115–122 | Cite as

Occurrence of nitrate in vegetables and dietary exposure assessment for the Cypriot adolescent consumers

  • Georgios Stavroulakis
  • Demetris Kafouris
  • Maria Christofidou
  • Lefkios Paikousis
  • Eftychia Christou
  • Maro Christodoulidou
  • Popi Kanari
  • Eleni Ioannou-Kakouri
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Abstract

In this study, a simple, rapid and sensitive ion chromatography method was used for the determination of mass fraction nitrate (mg kg−1) in 396 samples of vegetables. The method performance characteristics were determined after spiking blank samples. The mean recoveries in vegetables ranged from 94.0 % to 108.7 %. Limits of detection and quantification were 10 mg kg−1 and 30 mg kg−1, respectively. The results showed the mass fraction nitrate in vegetables was in the range of 10 mg kg−1–5619 mg kg−1. Leafy, root and stem vegetables, and herbs were the most contaminated, compared to other vegetables. Approximately 87 % of the samples were contaminated with nitrate; however, none of the samples analyzed was above the maximum limits for nitrate in vegetables. The dietary nitrate intake through consumption of vegetables was estimated for the adolescent population in Cyprus. The mean and 95th percentile intake values, based on median nitrate concentrations, were 1.12 kg d−1 and 3.42 mg kg−1 d−1, respectively. A small percentage (3.3 %) of the adolescent population had a nitrate intake above the Acceptable Daily Intake (3.7 mg kg−1 d−1). Lettuce and other leafy vegetables (spinach, rucola, purslane, beet leaves) contributed nearly 60 % to the overall nitrate exposure (mg kg−1 d−1).

Keywords

Nitrate Vegetables Ion chromatography Dietary exposure 

Notes

Acknowledgements

The authors gratefully acknowledge the Cyprus Government and Ministry of Health for financial support.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. 1.
    Walker R (1990) Nitrates, nitrites and N-nitrosocompounds: a review of the occurrence in food and diet and the toxicological implications. Food Addit Contam 7:717–768CrossRefGoogle Scholar
  2. 2.
    Gangolli SD, van den Brandt P, Feron V, Janzowsky C, Koeman J, Speijers G, Speigelhalder B, Walker R, Winshnok J (1994) Assessment of nitrate, nitrite, and N-nitroso compounds. Eur J Pharmacol Environ Toxicol Pharmacol Sect 292:1–38CrossRefGoogle Scholar
  3. 3.
    Worthington V (2001) Nutritional quality of organic versus conventional fruits, vegetables and grains. J Altern Complement Med 7(2):161–173CrossRefGoogle Scholar
  4. 4.
    Fytianos K, Zagoriannis P (1999) Nitrate and nitrite accumulation in fresh vegetables from Greece. Bull Environ Contam Toxicol 62:187–192CrossRefGoogle Scholar
  5. 5.
    Commission Regulation (EC) No. 1881/2006 of 19 December 2006 setting maximum levels for certain contaminants in foodstuffs, OJ L 364, 20 December 2006, p 5Google Scholar
  6. 6.
    Official Methods of Analysis (2005) 18th Ed., AOAC INTERNATIONAL, Gaithersburg, MD, Method 993.03Google Scholar
  7. 7.
    Moorcroft MJ, Davis J, Compton RG (2001) Detection and determination of nitrate and nitrite: a review. Talanta 54:785–803CrossRefGoogle Scholar
  8. 8.
    Gennaro MC, Bertolo PL (1989) Determination of the principal anionic components in wines and soft drinks by ion interaction reversed-phase high-performance liquid chromatography. J Chromatogr A 472:433–440CrossRefGoogle Scholar
  9. 9.
    Cheng CF, Tsang CW (1998) Simultaneous determination of nitrite, nitrate and ascorbic acid in canned vegetable juices by reverse-phase ion-interaction HPLC. Food Addit Contam 15:753–758CrossRefGoogle Scholar
  10. 10.
    Chou S, Chung JC, Hwang DF (2003) A high performance liquid chromatography method for determining nitrate and nitrite levels in vegetables. J Food Drug Anal 11:233–238Google Scholar
  11. 11.
    Bosch NB, Mata MG, Peñuela MJ, Galán TR, Ruiz BL (1995) Determination of nitrite levels in refrigerated and frozen spinach by ion chromatography. J Chromatogr A 706:221–228CrossRefGoogle Scholar
  12. 12.
    Siu DC, Henshall A (1998) Ion chromatographic determination of nitrate and nitrite in meat products. J Chromatogr A 804:157–160CrossRefGoogle Scholar
  13. 13.
    Ellis G, Adatia I, Yazdanpanah M, Makela S (1998) Nitrite and nitrate analysis: a clinical biochemistry perspective. Clin Biochem 31:195–220CrossRefGoogle Scholar
  14. 14.
    Ximenes M, Rath S, Reyes F (2000) Polarographic determination of nitrates in vegetables. Talanta 51:49–56CrossRefGoogle Scholar
  15. 15.
    Jimidar M, Hartmann C, Cousement N, Massart DL (1995) Determination of nitrate and nitrite in vegetables by capillary electrophoresis with indirect detection. J Chromatogr A 706:479–492CrossRefGoogle Scholar
  16. 16.
    Santamaria P (2006) Nitrate in vegetables: toxicity, content, intake and EC regulation. J Sci Food Agric 86:10–17CrossRefGoogle Scholar
  17. 17.
    Mensinga TT, Speijers GJA, Meulenbelt J (2003) Health implications of exposure to environmental nitrogenous compounds. Toxicol Rev 22:41–51CrossRefGoogle Scholar
  18. 18.
    Commission Regulation (EC) No 1882/2006 of 19 December 2006 laying down methods of sampling and analysis for the official control of the levels of nitrates in certain foodstuffs, OJ L 364, 20 December 2006, p 25–31Google Scholar
  19. 19.
    European Food Safety Authority (2011) Use of the EFSA comprehensive European food consumption database in exposure assessment. EFSA J 9(3):2097CrossRefGoogle Scholar
  20. 20.
    EFSA Panel on Contaminants in the Food Chain (CONTAM) (2010) Scientific Opinion on possible health risks for infants and young children from the presence of nitrates in leafy vegetables. EFSA J 8(12):1935Google Scholar
  21. 21.
    Casanova JA, Gross LK, Mcmullen SE, Schenck FJ (2006) Use of griess reagent containing vanadium(III) for post-column derivatization and simultaneous determination of nitrite and nitrate in baby food. J AOAC Int 89(2):447–451Google Scholar
  22. 22.
    Tamme T, Reinik M, Roasto M, Juhkam K, Tenno T, Kiis A (2006) Nitrates and nitrites in vegetables and vegetable-based products and their intakes by the Estonian population. Food Addit Contam 23(4):355–361CrossRefGoogle Scholar
  23. 23.
    Chung SY, Kim JS, Kim M, Hong MK, Lee JO, Kim CM, Song IS (2003) Survey of nitrate and nitrite contents of vegetables grown in Korea. Food Addit Contam 20(7):621–628CrossRefGoogle Scholar
  24. 24.
    De Martin S, Restani P (2003) Determination of nitrates by a novel ion chromatographic method: occurence in leafy vegetables (organic and conventional) and exposure assessment for Italian consumers. Food Addit Contam 20(9):787–792CrossRefGoogle Scholar
  25. 25.
    Temme EH, Vandevijvere S, Vinkx C, Huybrechts I, Goeyens L, Van Oyen H (2011) Average daily nitrate and nitrite intake in the Belgian population older than 15 years. Food Addit Contam 28(9):1193–1204CrossRefGoogle Scholar
  26. 26.
    Escobar-Gutierrez AJ, Burns IG, Lee A, Edmondson RN (2002) Screening lettuce cultivars for low nitrate content during summer and winter production. J Hortic Sci Biotechnol 7(2):232–237CrossRefGoogle Scholar
  27. 27.
    Pardo-Marín O, Yusà-Pelechà V, Villalba-Martín P, Perez-Dasí JA (2010) Monitoring programme on nitrates in vegetables and vegetable-based baby foods marketed in the Region of Valencia, Spain: levels and estimated daily intake. Food Addit Contam 27(4):478–486CrossRefGoogle Scholar
  28. 28.
    Nowrouz P, Taghipour H, Dastgiri S, Bafandeh Y, Hashemimajd K (2012) Nitrate determination of vegetables in Varzeghan City, North-western Iran. Health Promot Perspect 2(2):244–250Google Scholar
  29. 29.
    Correia M, Barroso A, Barroso MF, Soares D, Oliveira MBPP, Delerue-Matos C (2010) Contribution of different vegetable types to exogenous nitrate and nitrite exposure. Food Chem 120(4):960–966CrossRefGoogle Scholar
  30. 30.
    Ioannou-Kakouri E, Aletrari M, Christou E (2006) Risk assessment of the dietary intake of lead, cadmium, mercury and nitrates in Cyprus and the relevant uncertainty. http://www.moh.gov.cy/Moh/fsc/fsc.nsf/All/0AD54F14D78FA3C8C2257A93003A8387/$file/Risk%20assessment%20of%20lead,%20cadmium,%20mercury%20and%20nitrates.pdf. Accessed 14 July 2017
  31. 31.
    Menard C, Heraud F, Volatier JL, Leblanc JC (2008) Assessment of dietary exposure of nitrate and nitrite in France. Food Addit Contam 25(8):971–988CrossRefGoogle Scholar
  32. 32.
    FSANZ (Food Safety Authority of New Zealand) (2011) Survey of nitrates and nitrites in food and beverages in Australia. http://www.foodstandards.gov.au/science/surveillance/pages/surveyofnitratesandn5368.aspx. Accessed 6 July 2017
  33. 33.
    Suomi J, Ranta J, Tuominen P, Hallikainen A, Putkonen T, Bäckman C, Ovaskainen M-L, Virtanen S, Savela K (2013) Quantitative risk assessment on dietary exposure of children and adults to nitrate and nitrite. Evira Research Reports 2/2013. Helsinki: Finnish Food Safety Authority Evira. https://www.evira.fi/globalassets/tietoa-evirasta/julkaisut/julkaisusarjat/elintarvikkeet/kvantitatiivinen-riskinarviointi-lasten-ja-aikuisten-altistumisesta-nitraatille-ja-nitriitille.pdf. Accessed 21 July 2017
  34. 34.
    EFSA, Scientific Panel on Contaminants in the Food Chain (CONTAM) (2008) Opinion of the scientific panel on contaminants in the food chain on a request from the European Commission to perform a scientific risk assessment on nitrate in vegetables. EFSA J 689:1–79Google Scholar
  35. 35.
    EFSA ANS Panel (EFSA Panel on Food Additives and Nutrient Sources added to Food), Mortensen A et al (2017) Scientific Opinion on the re-evaluation of sodium nitrate (E 251) and potassium nitrate (E 252) as food additives. EFSA J 15(6):4787Google Scholar
  36. 36.
    Hong Kong - Centre for Food Safety (CFS) (2010) Nitrate and nitrite in vegetables available in Hong Kong, food and environmental hygiene department, the government of the Hong Kong special administrative region. http://www.cfs.gov.hk/english/programme/programme_rafs/files/Nitrate_and_Nitrite_Vegetables_Available_HK_e.pdf. Accessed 20 July 2017
  37. 37.
    EFSA (2006) Guidance of the Scientific Committee on a request from EFSA related to Uncertainties in Dietary Exposure Assessment. EFSA J 438:1–54Google Scholar
  38. 38.
    Ekart K, Hmelak Gorenjak A, Madorran E, Lapajne S, Langerholc T (2013) Study on the influence of food processing on mass fraction nitrate in vegetables. EFSA supporting publication 2013: EN-514 https://www.efsa.europa.eu/en/supporting/pub/en-514. Accessed 26 July 2017

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Georgios Stavroulakis
    • 1
  • Demetris Kafouris
    • 1
  • Maria Christofidou
    • 1
  • Lefkios Paikousis
    • 2
  • Eftychia Christou
    • 1
  • Maro Christodoulidou
    • 1
  • Popi Kanari
    • 1
  • Eleni Ioannou-Kakouri
    • 1
  1. 1.State General LaboratoryMinistry of HealthNicosiaCyprus
  2. 2.ImprovastNicosiaCyprus

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