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Amino Acids

, Volume 18, Issue 3, pp 239–249 | Cite as

The effects of thiopentone on free intracellular amino acids in polymorphonuclear leucocytes

  • J. Mühling
  • V. Knülle
  • M. Fuchs
  • M. G. Dehne
  • S. Weiss
  • G. Hempelmann

Summary.

Previous studies have shown the inhibitory effects of thiopentone on polymorphonuclear leucocyte (PML) function. However, major biochemical mechanisms which have been involved are still unknown. The aim of this study was therefore to investigate thiopentone's effects on intracellular amino acid metabolism in PML using both advanced PML separation – and HPLC techniques, especially developed for this purpose and precisely validated in our institute. Overall, our study indicates important dose-dependent alterations of free intracellular amino acid metabolism following thiopentone treatment and draw attention to the biochemical mechanisms which may be involved in both thiopentone-induced modulation in PML function and cellular immunocompetence.

Keywords: Amino acids – Leucocytes – Thiopentone – Intracellular amino acids – High-performance liquid chromatography – Amino acid metabolism 

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Copyright information

© Springer-Verlag Wien 2000

Authors and Affiliations

  • J. Mühling
    • 1
  • V. Knülle
    • 1
  • M. Fuchs
    • 2
  • M. G. Dehne
    • 1
  • S. Weiss
    • 1
  • G. Hempelmann
    • 1
  1. 1. Department of Anaesthesiology and Intensive Care Medicine, Justus-Liebig-University, Giessen, Federal Republic of GermanyDE
  2. 2. Firma Dr. Ing. Herbert Knauer GmbH, Berlin, Federal Republic of GermanyDE

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