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Amino Acids

, Volume 18, Issue 3, pp 207–217 | Cite as

Anaerobic accumulation of amino acids in rice roots: role of the glutamine synthetase/glutamate synthase cycle

  • R. Reggiani
  • M. Nebuloni
  • M. Mattana
  • I. Brambilla

Summary.

Accumulation of amino acids was studied in rice roots of 3-day-old seedlings subjected for 48 h to anaerobic conditions. Alanine and Gaba were the main amino acids accumulated under anoxia. Their synthesis was strongly inhibited by MSX and AZA, inhibitors of glutamine synthetase and glutamate synthase. These activities increased after 8 h of anaerobic treatment and, by immunoprecipitation of 35S-labeled proteins, it was shown that glutamine synthetase and ferredoxin-dependent glutamate synthase were synthesized during the treatment. These findings indicate that the glutamine synthetase/glutamate synthase cycle play an important role in anaerobic amino acid accumulation.

Keywords: Amino acids – Anoxia – Glutamate synthase – Glutamine synthetase – Rice – Root 

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Copyright information

© Springer-Verlag Wien 2000

Authors and Affiliations

  • R. Reggiani
    • 1
  • M. Nebuloni
    • 1
  • M. Mattana
    • 1
  • I. Brambilla
    • 1
  1. 1.Istituto Biosintesi Vegetali, CNR, Milano, ItalyIT

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