Abstract
This study aimed to evaluate the effect of taurine (tau) supplementation to low fishmeal (FM) diets on growth performance, oxidative status, and immune response of European seabass juveniles. Four isoproteic (46% crude protein) and isolipidic (19% crude lipid) diets were formulated to contain either 25 or 12.5% FM and a mixture of plant feedstuffs, supplemented or not with 1% tau. Twelve groups of 20 fish (IBW = 9.4 g) were fed each diet for 9 weeks. Reduction of dietary FM from 25 to 12.5% impaired growth performance, feed efficiency, and protein efficiency ratio but had no effect on nitrogen retention (% N intake). Independently of FM level, dietary tau supplementation improved growth performance and nitrogen retention without affecting feed efficiency. Dietary FM level reduction increased liver G6PDH activity, but did not affect lipid peroxidation or activities of redox key enzymes. Contrarily, dietary tau supplementation decreased hepatic G6PDH and GPX activities and lipid peroxidation. Gene expression COX-2 was not affected either by FM or tau levels but TNF-α increased with the reduction of FM level but not with the tau level. Dietary tau supplementation decreased Casp3 and Casp9 expression regardless of dietary FM level. Overall, this study evidenced that dietary tau supplementation improved growth performance and antioxidant response and reduced intestine inflammatory and apoptosis processes.
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This work was supported by the Structured R&D&I Project INNOVMAR—Innovation and Sustainability in the Management and Exploitation of Marine Resources (ref. NORTE-01-0145-FEDER-000035) within the research line “INSEAFOOD—Innovation and valorization of seafood products: meeting local challenges and opportunities”, founded by the Northern Regional Operational Programme (NORTE2020) through the European Regional Development Fund (ERDF). N. Martins was supported by INSEAFOOD Grant Inseafood/BI/L2/2017-026, R. Magalhães was supported by FCT Grant SFRH/BD/11570/2016, C. Castro was supported by FCT Grant SFRH/BPD/114942/2016, A. Couto was supported by National funds through FCT.
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Martins, N., Magalhães, R., Castro, C. et al. Taurine modulates hepatic oxidative status and gut inflammatory markers of European seabass (Dicentrarchus labrax) fed plant feedstuffs-based diets. Amino Acids 51, 1307–1321 (2019). https://doi.org/10.1007/s00726-019-02769-4
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DOI: https://doi.org/10.1007/s00726-019-02769-4