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Surfactin application for a short period (10/20 s) increases the surface wettability of sound dentin

  • Larissa Marcelino
  • Julia Puppin-Rontani
  • François Coutte
  • M. Terêsa Machini
  • Augusto EtchegarayEmail author
  • Regina Maria Puppin-Rontani
Short Communication
  • 75 Downloads

Abstract

The aim of this study was to evaluate the effect of spreading the lipopeptide surfactin, for short time (10/20 s), on dentin wettability. Study groups were surfactin: 2.8; 1.4; 0.7; 0.35; and 0.175 mg/mL and a control group that received no treatment. Dentin discs (4 mm height) were prepared and polished with 600-grit SiC paper. Contact angle determinations were carried out after microbrush spreading of surfactin on dentin specimens for, respectively, 10 and 20 s. Excess liquid was removed, and after 60 s, the specimens were analyzed in a goniometer using the sessile drop method to measure the contact angle. Results were analyzed by two-way ANOVA (concentration × time) and t student, with α = 0.05. Lower contact angles were obtained for surfactin (0.7 mg/mL) spread for 10 s. However, no statistical difference was observed for surfactin (2.8 mg/mL) applied during 20 s. Higher contact angles were observed for surfactin (0.7 mg/mL) spread for 20 s. In conclusion, dentin wettability is dependent on spreading time and surfactin concentration.

Keywords

Dentin Contact angle Wettability Surfactin 

Notes

Acknowledgements

This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001.– (Master's scholarship to Marcelino, L) and by the Fundação de Amparo à Pesquisa de São Paulo (Fapesp 2015/14360-4, to Machini, MT). The authors would like to thank Dr. Cleber W. Liria for the help in mass spectrometry analysis and Dr. Sérgio L. Pinheiro for important suggestions to this study.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Ethical approval

This work was approved by the Ethics Committee in Research with Humans of the Pontifical Catholic University of Campinas, under the number 2.907.654.

Informed consent

The free informed consent form for specimen donation was signed by all participants in this research.

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Center for Life Sciences, Postgraduate Program in Health SciencesPontifical Catholic University of Campinas (PUC-Campinas)CampinasBrazil
  2. 2.Department of Operative Dentistry, Piracicaba Dental SchoolUniversity of CampinasPiracicabaBrazil
  3. 3.Department of Pediatric Dentistry, Piracicaba Dental SchoolUniversity of CampinasPiracicabaBrazil
  4. 4.Univ. Lille, INRA, ISA, Univ. Artois, Univ. Littoral Côte d’Opale, EA 7394-ICV Institut Charles ViolletteLilleFrance
  5. 5.Department of Biochemistry, Institute of ChemistryUniversity of São PauloSão PauloBrazil

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