Abstract
The aim of this study was to evaluate the effect of spreading the lipopeptide surfactin, for short time (10/20 s), on dentin wettability. Study groups were surfactin: 2.8; 1.4; 0.7; 0.35; and 0.175 mg/mL and a control group that received no treatment. Dentin discs (4 mm height) were prepared and polished with 600-grit SiC paper. Contact angle determinations were carried out after microbrush spreading of surfactin on dentin specimens for, respectively, 10 and 20 s. Excess liquid was removed, and after 60 s, the specimens were analyzed in a goniometer using the sessile drop method to measure the contact angle. Results were analyzed by two-way ANOVA (concentration × time) and t student, with α = 0.05. Lower contact angles were obtained for surfactin (0.7 mg/mL) spread for 10 s. However, no statistical difference was observed for surfactin (2.8 mg/mL) applied during 20 s. Higher contact angles were observed for surfactin (0.7 mg/mL) spread for 20 s. In conclusion, dentin wettability is dependent on spreading time and surfactin concentration.
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Acknowledgements
This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001.– (Master's scholarship to Marcelino, L) and by the Fundação de Amparo à Pesquisa de São Paulo (Fapesp 2015/14360-4, to Machini, MT). The authors would like to thank Dr. Cleber W. Liria for the help in mass spectrometry analysis and Dr. Sérgio L. Pinheiro for important suggestions to this study.
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Marcelino, L., Puppin-Rontani, J., Coutte, F. et al. Surfactin application for a short period (10/20 s) increases the surface wettability of sound dentin. Amino Acids 51, 1233–1240 (2019). https://doi.org/10.1007/s00726-019-02750-1
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DOI: https://doi.org/10.1007/s00726-019-02750-1