Abstract
Sus scrofa provides a major source of animal protein for humans as well as being an excellent biomedical model. This study was carried out to understand, in detail, the genetic and functional variants of Jeju Native Pigs and miniature pigs through differential expression profiling of the genes controlling their immune response, growth performance, and meat quality. The Illumina HiSeq 2000 platform was used for generating 1.3 billion 90 bp paired-end reads, which were mapped to the S. scrofa genome using TopHat2. A total of 2481 and 2768 genes were differentially expressed with 8-log changes in muscle and liver samples, respectively. Five hundred forty-eight genes in muscle and 642 genes in liver samples had BLAST matches within the non-redundant database. GO process and pathway analyses showed enhanced biological processes related to the extracellular structural organization and skeletal muscle cell differentiation in muscle tissue, whereas the liver tissue shares functions related to the inflammatory response. Herein, we identify inflammatory regulatory genes in miniature pigs and growth response genes in Jeju Native Pigs, information which can provide a stronger base for the selection of breeding stock and facilitate further in vitro and in vivo studies for therapeutic purposes.
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Abbreviations
- AM:
-
Alveolar macrophage
- AMBP :
-
Alpha-1-microglobulin/bikunin precursor
- ACP5 :
-
Acid phosphatase 5
- COL21A1 :
-
Collagen type XXI alpha 1
- CXCL13 :
-
C-X-C Motif Chemokine Ligand 13
- DAVID:
-
Database for Annotation, Visualization and Integrated Discovery
- DEGs:
-
Differentially expressed genes
- FABPs:
-
Fatty acid-binding proteins
- FABP6 :
-
Fatty acid-binding protein 6
- FDR:
-
False discovery rate
- GO:
-
Gene ontology
- JNP:
-
Jeju Native Pig
- KEGG:
-
Kyoto encyclopedia of genes and genomes
- MyBPH :
-
Myosin binding protein H
- MARCO :
-
Macrophage receptor with collagenous
- NGS:
-
Next-generation sequencing
- NPG3 :
-
Protegrin 3
- PCA:
-
Principal component analysis
- PC:
-
Principal component
- POSTN :
-
Periostin
- PNLIP :
-
Pancreatic lipase
- PNPLA3 :
-
Patatin-like phospholipase domain-containing protein 3
- SPENCD:
-
Spondyloenchondrodysplasia
- SPENCDI:
-
SPENCD with immune dysregulation
- SVs:
-
Single variations
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Acknowledgements
This study was supported by a grant from the Next-Generation BioGreen 21 Program (No. PJ01316701; PJ01117401, and PJ0099272014), Rural Development Administration, Republic of Korea, hence the authors are thankful for this organization. Author MG also thankful to Lovely Professional University for provide necessary requirements.
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DKJ and MG contributed to the design the conception and designed the project; MG contributed to design, analysis, and interpretation of data; MG, NK, and JZ contributed to the collected, harvested and mRNA analyses; DCK, DH, KTD and TK contributed to collect the field samples; DKJ, KD contributed to the evaluation of intellectual contents; KKP contributed to further literature corrections; DKJ and NS contributed to revising the manuscript critically for important intellectual content; HKL and KDS contributed to the additional validation and data analysis; DKJ contributed to the final approval of the manuscript. All authors provided critical input in manuscript completion and approved the final version of the manuscript.
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All procedures performed in this experiment were approved by the regional Ethical Committee (Jeju National University Animal Bioethics committee permit number: 2013-0009). The animals were handled in accordance with proper animal welfare guidelines.
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Written and intellectual contents of this article were obtained from all the authors listed in the study. All authors have been contributed to conception, design, interpretation of data and analysis.
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Ghosh, M., Sharma, N., Gera, M. et al. The first comprehensive description of the expression profile of genes involved in differential body growth and the immune system of the Jeju Native Pig and miniature pig. Amino Acids 51, 495–511 (2019). https://doi.org/10.1007/s00726-018-2685-5
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DOI: https://doi.org/10.1007/s00726-018-2685-5