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Amino Acids

, Volume 51, Issue 2, pp 331–343 | Cite as

The first identification and characterization of a histidine-specific amino acid racemase, histidine racemase from a lactic acid bacterium, Leuconostoc mesenteroides subsp. sake NBRC 102480

  • Motoyasu Adachi
  • Rumi Shimizu
  • Shiro Kato
  • Tadao OikawaEmail author
Original Article

Abstract

We expressed a histidine racemase from Leuconostoc mesenteroides subsp. sake NBRC 102480 (Lm-HisR) successively in a soluble fraction of Escherichia coli BL21 (DE3) and then highly purified it from the cell-free extract. Lm-HisR showed amino acid racemase activity on histidine specifically. This is the first example of an amino acid racemase specifically acting on histidine. Phylogenetic analysis of Lm-HisR showed that Lm-HisR was located far from the cluster of alanine racemases reported thus far and only in lactic acid bacteria of the genus Leuconostoc. Alignment of the primary structure of Lm-HisR with those of lysine and alanine racemases and alanine racemase homologs previously reported revealed that the PLP-binding lysine and catalytic tyrosine were completely conserved, and some residues that are unique to the phylogenetic branch of Lm-HisR, Phe44, Ser45, Thr174, Thr206, His286, Ser287, Phe292, Gly312, Val357, and Ala358 were identified. We determined the crystal structure of Lm-HisR complexed with PLP at a 2.1-Å resolution. The crystal structure contained four molecules (two dimers) in the asymmetric unit. When comparing the 3D structure of Lm-HisR with those of racemases from Geobacillus stearothermophilus and Oenococcus oeni, Met315 was completely conserved, but Val357 was not. In addition, two significant differences were observed between Lm-HisR and G. stearothermophilus alanine racemase. Phe44 and His286 in Lm-HisR corresponded to Tyr43 and Tyr284 in G. stearothermophilus alanine racemase, respectively. Based on the structural analysis, comparison with alanine racemase, and docking simulation, three significant residues, Phe44, His286, and Val357, were identified that may control the substrate specificity of Lm-HisR.

Keywords

Leuconostoc mesenteroides Histidine racemase d-Histidine Amino acid racemase X-ray crystallographic structure 

Abbreviations

ASA

Accessible surface area

Lm-HisR

Histidine racemase from Leuconostoc mesenteroides subsp. sake NBRC 102480

PDB

Protein data bank

PLP

Pyridoxal 5´-phosphate

RMSD

Root mean square deviations

Notes

Acknowledgements

The synchrotron radiation experiments were performed on the BL5A beamline at Photon Factory (proposal no. 2014G645). This work was also supported by JSPS KAKENHI grant number JP16KT0063 (awarded to MA and TO). This study was supported in part by the Ministry of Education, Culture, Sports, Science and Technology (MEXT), the Support Program for the Strategic Research Foundation at Private Universities (2013–2017), and the Kansai University Organization for Research and Development of Innovative Science and Technology (ORDIST) Grant (2018). We thank Dr. Yoshitaka Gogami for the construction of pET21b-Lm-hisR and characterization of the basic properties of Lm-HisR. Finally, we appreciate the contribution of Dr. Ryota Kuroki to promote in the early stages of this study.

Author contributions

TO designed the studies. TO, MA, and SK wrote the paper. RS and SK conducted the experiments. TO and SK conducted the phylogenetic analysis. MA analyzed the X-ray crystal structure.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This manuscript does not contain any studies with human participants or animals performed by any of the authors.

Supplementary material

726_2018_2671_MOESM1_ESM.pptx (106 kb)
Supplementary material 1 (PPTX 105 kb)
726_2018_2671_MOESM2_ESM.pptx (60 kb)
Supplementary material 2 (PPTX 59 kb)

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  • Motoyasu Adachi
    • 3
  • Rumi Shimizu
    • 3
  • Shiro Kato
    • 2
    • 4
  • Tadao Oikawa
    • 1
    • 2
    Email author
  1. 1.Department of Life Science and Biotechnology, Faculty of Chemistry, Materials and BioengineeringKansai UniversitySuitaJapan
  2. 2.Kansai University High Technology Research CenterSuitaJapan
  3. 3.Tokai Quantum Beam Science Center, Takasaki Advanced Radiation Research InstituteNational Institutes for Quantum and Radiological Science and TechnologyTokaiJapan
  4. 4.International Institute of Rare Sugar Research and EducationKagawa UniversityMikiJapan

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