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Amino Acids

, Volume 51, Issue 2, pp 311–318 | Cite as

Isolation and antiproliferation of tumor cells by a novel peptide (TC22) from the beetle Tribolium castaneum

  • Xiao-dong LiuEmail author
  • Fu-xin Zhang
  • Zhi-hua Qin
  • Hu ShanEmail author
Original Article

Abstract

Anticancer peptides (ACPs) are biologically anticancer active molecules that are produced by mammals, plants, insects and microorganisms. Here, a new peptide (TC22) with the amino acid sequence MTVVLLLIVLPLLGGVHSSGIL was identified and characterized from the beetle Tribolium castaneum. We found it inhibited the growth and viability of HeLa and MCF-7 cells. Flow cytometry analysis demonstrated the TC22 induced HeLa cell apoptosis, and activated caspase-9 and caspase-3. Furthermore, TC22 led to ROS generation, and triggered p53 transcription and expression. Taken together, our results indicated that TC22 exhibited high anticancer capacity via activating p53, inducing ROS generation and through a mitochondrial pathway. This research provided a novel natural source peptide with strong anticancer capacity. These findings provide some novel insights on the potential candidate reagent in cancer treatment.

Keywords

Tribolium castaneum Anticancer peptide Apoptosis 

Notes

Acknowledgements

The authors are grateful to the Research Fund for the Qingdao applied basic research project (16-5-1-53-jch), the National Natural Science Foundation (No. 31502044), the Priority Academic Talent Team Cultivation Program of the colleges and universities in Shandong Province, and the Doctoral Program of Qingdao Agriculture University (No. 663/1115001).

Author contributions

XL and HS conceived and designed the experiments. XL, FZ, ZQ performed the experiments. XL and FZ analyzed the data. XL wrote the manuscript. Other authors provided editorial advices.

Compliance with ethical standards

Conflict of interest

The authors declare no competing financial interest.

Ethical standard

This article does not contain any studies with human participants or animals performed by any of the authors.

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  1. 1.College of Veterinary MedicineQingdao Agricultural UniversityQingdaoChina

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