PGC1α regulates ACMSD expression through cooperation with HNF4α
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ACMSD is a tryptophan metabolic key enzyme. HNF4α regulates the transcription of some energy-metabolic enzymes by cooperating with PGC1α, a major transcriptional co-regulator involved in energy metabolism. In this study, we investigated the involvement of PGC1α in Acmsd expression through cooperation with HNF4α. Luciferase reporter assay was performed in NIH3T3 cells using a reporter vector containing HNF4α responsive elements in the Acmsd 5′ upstream transcriptional regulatory region together with HNF4α and/or PGC1α expression vectors. The Acmsd luciferase reporter activity was greatly elevated by co-overexpression of HNF4α and PGC1α in NIH3T3 cells. Moreover, the expression level of Acmsd mRNA was significantly increased by co-overexpression of HNF4α and PGC1α in primary hepatocytes compared with expression of either HNF4α or PGC1α alone. These results indicate that PGC1α is involved in Acmsd expression through cooperation with HNF4α.
KeywordsACMSD PGC1α HNF4α Quinolinate Tryptophan NAD Nicotinamide
This work was supported in part to Y.E. by a Grant-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan and Initiative for Realizing Diversity in the Research Environment of Chiba University.
Compliance with ethical standards
Conflict of interest
The authors declare that we have no conflict of interest.
The care and treatment of mice were in accordance with protocols approved by the local institutional guidelines for animal care of Chiba University and complied with the “Guide for the care and Use of Laboratory Animals” (NIH publication no. 85-23, revised 1985).
Human and animal rights statement
This article does not contain any studies with human participants performed by any of the authors. Animal experiments were performed according to the guidelines of the National Institutes of Health and the institutional rules for the use and care of laboratory animals at Chiba University.
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