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Amino Acids

, Volume 50, Issue 12, pp 1729–1737 | Cite as

Dietary l-lysine supplementation altered the content of pancreatic polypeptide, enzymes involved in glutamine metabolism, and β-actin in rats

  • Chao-Wu XiaoEmail author
  • Caroline Faddoul
  • Carla Wood
Original Article

Abstract

This study investigated the effects of Lys supplementation on serum pancreatic polypeptide (PP), glutamine (Gln) levels and the expression of PP, Gln synthetase (GlnS), glutaminase (Gls) and β-actin in different tissues such as pancreas, skeletal muscle, liver and kidney in rats. Male Sprague–Dawley rats were fed diets containing 7% casein supplemented with either 0% (Control), 1%, 1.5%, 3% Lys or 3% Lys with 1.5% Arg for a week. All rats were necropsied for collection of blood and tissues. Expression of PP, GlnS, Gls, and β-actin in tissues were determined using Western blotting. The results showed that the rats fed 3% supplemental Lys had significantly lower body weight gain (BWG) and food intake than the ≤ 1.5% Lys groups (P < 0.05). Supplementation with ≥ 1% Lys increased serum PP level (P < 0.05), but had no significant effect on pancreatic PP abundance (P > 0.05). GlnS expression was significantly lowered in skeletal muscle by ≥ 1.5% supplemental Lys compared to the Control (P < 0.05). The expression of Gls in the kidney was increased by the addition of 1.5% Arg to 3% Lys diet (P < 0.05). Liver β-actin significantly increased with both Lys and Arg supplementation and muscle β-actin significantly decreased (P < 0.05) with ≥ 1.5% supplemental Lys. Kidney β-actin significantly increased with Arg supplementation vs 3% Lys alone (P < 0.05). These results showed that dietary supplementation with ≥ 1.5% Lys significantly suppressed GlnS expression in the skeletal muscle, which may contribute to the decreased serum Gln levels, and that increased serum PP by Lys may be due to suppressed catabolism rather than increased synthesis of PP. Lys-induced PP may play a role in reducing food intake and BWG.

Keywords

l-Lysine supplementation l-Arginine Pancreatic polypeptide Glutamine β-Actin Rats 

Abbreviations

AA

Amino acid

Arg

l-Arginine

BWG

Body weight gain

Gln

Glutamine

GlnS

Gln synthetase

Gls

Glutaminase

Lys

l-Lysine

PP

Pancreatic polypeptide

Notes

Acknowledgements

This research was funded by Health Canada.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Research involving animals

The animal experimental protocol (ACC#2016-006) was approved by the Health Canada-Ottawa Animal Care Committee, and all animal handling and care followed the guidelines of the Canadian Council for Animal Care.

Informed consent

All authors in this paper have read the final manuscript and approved for publication.

Supplementary material

726_2018_2648_MOESM1_ESM.pptx (90 kb)
Supplementary material 1 (PPTX 90 kb)

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Copyright information

© Crown 2018

Authors and Affiliations

  1. 1.Nutrition Research Division, Food Directorate, Health Products and Food BranchHealth Canada, Banting Research CentreOttawaCanada
  2. 2.Food and Nutrition Science Program, Department of ChemistryCarleton UniversityOttawaCanada

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