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Amino Acids

, Volume 50, Issue 12, pp 1679–1684 | Cite as

Nutritionally non-essential amino acids are dispensable for whole-body protein synthesis after exercise in endurance athletes with an adequate essential amino acid intake

  • Hiroyuki Kato
  • Kimberly A. Volterman
  • Daniel W. D. West
  • Katsuya Suzuki
  • Daniel R. MooreEmail author
Original Article

Abstract

The increased protein requirement of endurance athletes may be related to the need to replace exercise-induced oxidative losses, especially of the branched-chain amino acids (BCAA). However, it is unknown if non-essential amino acids (NEAA) influence the requirement for essential amino acids (EAA) during post-exercise recovery. Seven endurance-trained males ran 20 km prior to consuming [13C]phenylalanine, sufficient energy, and: (1) deficient protein (BASE); (2) BASE supplemented with sufficient BCAA (BCAAsup); (3) an equivalent EAA intake as BCAA (LowEAA), and; (4) sufficient EAA intake (HighEAA). [13C]Phenylalanine oxidation (the reciprocal of protein synthesis) for BCAAsup and HighEAA (0.54 ± 0.15, 0.49 ± 0.11 µmol kg−1 h−1; Mean ± SD) were significantly lower than BASE (0.74 ± 0.14 µmol kg−1 h−1; P < 0.01 for both) and LowEAA (0.70 ± 0.11 µmol kg−1 h−1; P < 0.05 and 0.01, respectively). Our results suggest that exogenous NEAA are dispensable for whole-body protein synthesis during recovery from endurance exercise provided sufficient EAA are consumed. Endurance athletes who may be at risk of not meeting their elevated protein requirements should prioritize the intake of EAA-enriched foods and/or supplements.

Keywords

Endurance training Amino acid requirement Post-exercise recovery Protein synthesis Non-essential amino acids 

Abbreviations

BASE

Low protein diet

BCAA

Branched-chain amino acids

BCAAsup

BASE supplemented branched-chain amino acids

EAA

Essential amino acid

EEE

Exercise-induced energy expenditure

F13CO2

13CO2 excretion

FFM

Fat-free mass

FM

Fat mass

HighEAA

High dose of essential amino acids

HR

Heart rate

IAAO

Indicator amino acid oxidation

LowEAA

Low dose of essential amino acids

NEAA

Non-essential amino acid

OX

Phenylalanine oxidation

Q

Whole-body phenylalanine flux

RDA

Recommended daily allowance

REE

Resting energy expenditure

VCO2

CO2 production

Notes

Author contribution

The authors’ responsibilities were as follows: HK and DRM designed the research; HK, KAV and DW conducted research and analyzed the data; HK performed the statistical analysis; HK and DRM wrote the manuscript with assistance from KAV and DW; KS consulted the study design and manuscript writing. HK and DRM had primary responsibility for final content. All authors read and approved the final manuscript.

Funding

This study was funded by Ajinomoto Co., Inc.

Compliance with ethical standards

Conflict of interest

Some of the authors (HK and KS) are employees of Ajinomoto. DRM has received research grant from Ajinomoto Co., Inc.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. This study was approved by the research ethics board of the University of Toronto (No. 33245) and the institutional review board of Ajinomoto Co., Inc (No. 2016-017). This trial was registered at clinicaltrial.gov as NCT02874638.

Informed consent

Informed consent was obtained from all individual participants included in the study, after informing the purpose of the study, the experimental procedures involved and all the potential risks involved.

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Copyright information

© Springer-Verlag GmbH Austria, part of Springer Nature 2018

Authors and Affiliations

  • Hiroyuki Kato
    • 1
    • 2
  • Kimberly A. Volterman
    • 2
  • Daniel W. D. West
    • 2
  • Katsuya Suzuki
    • 1
  • Daniel R. Moore
    • 2
    Email author
  1. 1.Frontier Research Laboratories, Institute for InnovationAjinomoto Co., IncKawasakiJapan
  2. 2.Faculty of Kinesiology and Physical EducationUniversity of TorontoTorontoCanada

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