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Functionality of an absolutely conserved glycine residue in the chimeric relaxin family peptide R3/I5

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Abstract

The insulin superfamily is a group of homologous proteins that are further divided into the insulin family and relaxin family according to their distinct receptors. All insulin superfamily members contain three absolutely conserved disulfide linkages and a nonchiral Gly residue immediately following the first B-chain cysteine. The functionality of this conserved Gly residue in the insulin family has been studied by replacing it with natural l-amino acids or the corresponding unnatural d-amino acids. However, such analysis has not been conducted on relaxin family members. In the present study, we conducted chiral mutagenesis on the conserved B11Gly of the chimeric relaxin family peptide R3/I5, which is an efficient agonist for receptor RXFP3 and RXFP4. Similar to the effects on insulin family foldability, l-Ala or l-Ser substitution completely abolished the in vitro refolding of a recombinant R3/I5 precursor; whereas, d-Ala or d-Ser substitution had no detrimental effect on refolding of a semi-synthetic R3/I5 precursor, suggesting that the conserved Gly residue controls the foldability of relaxin family members. In contrast to the effect on insulin family activity, d-Ala or d-Ser replacement had no detrimental effect on the binding and activation potencies of the mature R3/I5 towards both RXFP3 and RXFP4, suggesting that the conserved Gly residue is irrelevant to the relaxin family’s activity. The present study revealed functionality of the conserved B-chain Gly residue for a relaxin family peptide for the first time, providing an overview of its contribution to foldability and activity of the insulin superfamily.

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Acknowledgements

This work was supported by Grants from the National Natural Science Foundation of China (CN) (31670773, 31470767).

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Authors and Affiliations

  1. Research Center for Translational Medicine at East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai, China

    Jia-Hui Wang, Xiao-Xia Shao, Meng-Jun Hu, Ya-Li Liu, Zeng-Guang Xu & Zhan-Yun Guo

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  1. Jia-Hui Wang
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  2. Xiao-Xia Shao
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  3. Meng-Jun Hu
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  4. Ya-Li Liu
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  5. Zeng-Guang Xu
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  6. Zhan-Yun Guo
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Correspondence to Zhan-Yun Guo.

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Wang, JH., Shao, XX., Hu, MJ. et al. Functionality of an absolutely conserved glycine residue in the chimeric relaxin family peptide R3/I5. Amino Acids 51, 619–626 (2019). https://doi.org/10.1007/s00726-018-02694-y

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  • DOI: https://doi.org/10.1007/s00726-018-02694-y

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