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Evaluation of peptides release using a natural rubber latex biomembrane as a carrier

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Abstract

The biomembrane natural (NRL—Natural Rubber Latex), manipulated from the latex obtained from the rubber tree Hevea brasiliensis, has shown great potential for application in biomedicine and biomaterials. Reflecting the biocompatibility and low bounce rate of this material, NRL has been used as a physical barrier to infectious agents and for the controlled release of drugs and extracts. The aim of the present study was to evaluate the incorporation and release of peptides using a latex biomembrane carrier. After incorporation, the release of material from the membrane was observed using spectrophotometry. Analyses using HPLC and mass spectroscopy did not confirm the release of the antimicrobial peptide [W6]Hylin a1 after 24 h. In addition, analysis of the release solution showed new compounds, indicating the degradation of the peptide by enzymes contained in the latex. Additionally, the release of a peptide with a shorter sequence (Ac-WAAAA) was evaluated, and degradation was not observed. These results showed that the use of NRL as solid matrices as delivery systems of peptide are sequence dependent and could to be evaluated for each sequence.

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Funding

This study was funded by São Paulo Research Foundation—FAPESP—(Grant numbers 2011/17411-8, 2012/15346-7 and 2015/02343-8) and Conselho Nacional de Desenvolvimento Científico—CNPq (Grant numbers 301512/2015-9 and 470261/2012-9).

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Authors and Affiliations

  1. Instituto de Química, UNESP, Universidade Estadual Paulista, Rua Prof. Francisco Degni, 55, Araraquara, São Paulo, 14800-060, Brazil

    M. C. R. Miranda, F. A. Borges, N. R. Barros, N. A. Santos Filho, R. D. Herculano & E. M. Cilli

  2. Bioprocess and Biotechnology Department, FCF, UNESP, Universidade Estadual Paulista, Araraquara, São Paulo, Brazil

    M. C. R. Miranda, F. A. Borges, N. R. Barros & R. D. Herculano

  3. Ciências Biológicas Department, UFTM, Universidade Federal do Triângulo Mineiro, Uberaba, Minas Gerais, Brazil

    R. J. Mendonça

Authors
  1. M. C. R. Miranda
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  2. F. A. Borges
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  3. N. R. Barros
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  4. N. A. Santos Filho
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  5. R. J. Mendonça
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  6. R. D. Herculano
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  7. E. M. Cilli
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Corresponding authors

Correspondence to R. D. Herculano or E. M. Cilli.

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The authors declare that they have no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Informed consent was obtained from all individual participants included in the study.

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Handling Editor: M. S. Palma.

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Miranda, M.C.R., Borges, F.A., Barros, N.R. et al. Evaluation of peptides release using a natural rubber latex biomembrane as a carrier. Amino Acids 50, 503–511 (2018). https://doi.org/10.1007/s00726-017-2534-y

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  • DOI: https://doi.org/10.1007/s00726-017-2534-y

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