Abstract
This study evaluated the effect of three levels of digestible amino acids (DAA; 100, 107 and 114% of Cobb recommendations) on mRNA abundance of peptide (PepT1) and amino acid (AA) transporters in 480-day-old broilers during prestarter period. Jejunal mRNA levels of the PepT1 and b0,+AT increased as DAA level increased from 100 to 114%. The expression of CAT1 mRNA in the jejunum was higher in birds fed 100% DAA diet. The transport systems B0AT and y+LAT1 were not affected by the dietary treatments. These results demonstrated that dietary content of protein and DAA differentially affected the expression of intestinal peptide and AA transporters to modulate absorption of peptide and AA in broilers.
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References
Bröer S (2008) Amino acid transport across mammalian intestinal and renal epithelia. Physiol Rev 88:249–286
Chen H, Pan Y, Wong EA, Webb KE Jr (2005) Dietary protein level and stage of development affect expression of an intestinal peptide transporter (cPepT1) in chickens. J Nutr 135:193–198
Closs EI, Simon A, Vékony N, Rotmann A (2004) Plasma membrane transporters for arginine. J Nutr 134:2752S–2767S
Fafournoux P, Bruhat A, Jousse C (2000) Amino acid regulation of gene expression. Biochem J 351:1–12
Ferraris RP (2000) Intestinal transport during fasting and malnutrition. Annu Rev Nutr 20:195–219
Ferraris R, Diamond J (1989) Specific regulation of intestinal nutrient transporters by their dietary substrates. Annu Rev Physio 51:125–141
García-Villalobos H, Morales-Trejo A, Araiza-Piña BA, Htoo JK, Cervantes-Ramírez M (2012) Effects of dietary protein and amino acid levels on the expression of selected cationic amino acid transporters and serum amino acid concentration in growing pigs. Arch Anim Nutr 66(4):1–14
Gilbert ER, Li H, Emmerson DA, JrKE Webb, Wong EA (2008) Dietary protein quality and feed restriction influence abundance of nutrient transporter mRNA in the small intestine of broiler chicks. J Nutr 138:262–271
Gilbert ER, Li H, Emmerson DA, JrKE Webb, Wong EA (2010) Dietary protein composition influences abundance of peptide and amino acid transporter messenger ribonucleic acid in the small intestine of 2 lines of broiler chicks. Poult Sci 89:1663–1676
Hatzoglou M, Fernandez J, Yaman I, Closs E (2004) Regulation of cationic amino acid transport: the story of the CAT-1 transporter. Ann Rev Nutr 24:377–399
He LQ, Yang HS, Hou YQ, Li T, Fang J, Zhou X, Yin Y, Wu L, Nyachoti M, Wu G (2013) Effects of dietary l-lysine intake on the intestinal mucosa and expression of cat genes in weaned piglets. Amino Acids 45(2):383–391
Majumder M, Yaman I, Gaccioli F, Zeenko VV, Wang C, Caprara MG, Venema RC, Komar AA, Snider MD, Hatzoglou M (2009) The hnRNA-binding proteins hnRNP L and PTB are required for efficient translation of the Cat-1 arginine/lysine transporter mRNA during amino acid starvation. Mol Cell Biol 29:2899–2912
Morales A, Buenabad L, Castillo G, Arce N, Araiza BA, Htoo JK, Cervantes M (2015) Low-protein amino acid–supplemented diets for growing pigs: effect on expression of amino acid transporters, serum concentration, performance, and carcass composition. J Anim Sci 93:2154–2164
Morales A, Buenabad L, Castillo G, Vzquez L, Espinoza S, Htoo JK, Cervantes M (2017) Dietary levels of protein and free amino acids affect pancreatic proteases activities, amino acids transporters expression and serum amino acid concentrations in starter pigs. J Anim Physiol Anim Nutr 101(4):723–732
Pineda M, Wagner CA, Bröer A, Stehberger PA, Kaltenbach S, Gelpí JL, Martín Del Río R, Zorzano A, Palacín M, Lang F, Bröer S (2004) Cystinuria-specific rBAT(R365W) mutation reveals two translocation pathways in the amino acid transporter rBAT-b0,+AT. Biochem J 377:665–674
Shiraga T, Miyamoto KI, Tanaka H, Yamamoto H, Taketani Y, Morita K, Tamai I, Tsuji A, Takeda E (1999) Cellular and molecular mechanisms of dietary regulation on rat intestinal H+/peptide transporter PepT1. Gastroenterology 116:354–362
Walker D, Thwaites DT, Simmons NL, Gilbert HJ, Hirst BH (1998) Substrate upregulation of human small intestinal peptide transporter, hPEPT1. J Physiol 507:696–706
Wenzel U, Meissner B, Doèring F, Daniel H (2001) PepT1-mediated uptake of dipeptides enhances the intestinal absorption of amino acids via transport system b0,+. J Cell Physiol 186:251–259
Wu G (2009) Amino acids: metabolism, functions, and nutrition. Amino Acids 37:1–17
Wu L, He L, Cui Z, Liu G, Yao K, Wu F, Li J, Li T (2015) Effects of reducing dietary protein on the expression of nutrition sensing genes (amino acid transporters) in weaned piglets. J Zhejiang Univ Sci B 16(6):496–502
Zeng PL, Li XG, Wang XQ, Zhang DX, Shu G, Luo QB (2011) The relationship between gene expression of cationic and neutral amino acid transporters in the small intestine of chick embryos and chick breed, development, sex, and egg amino acid concentration. Poult Sci 90:2548–2556
Zhang S, Saremi B, Gilbert ER, Wong EA (2017) Physiological and biochemical aspects of methionine isomers and a methionine analogue in broilers. Poult Sci 96(2):425–439
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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. All procedures performed in the present study involving animals were done so in accordance with the ethical standards of the institution at which the present study was conducted.
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Osmanyan, A.K., Ghazi Harsini, S., Mahdavi, R. et al. Intestinal amino acid and peptide transporters in broiler are modulated by dietary amino acids and protein. Amino Acids 50, 353–357 (2018). https://doi.org/10.1007/s00726-017-2510-6
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DOI: https://doi.org/10.1007/s00726-017-2510-6