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β-Conglycinin enhances autophagy in porcine enterocytes

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Abstract

β-Conglycinin (β-CG) is well known for inducing intestinal allergies and dysfunction in neonates and young pigs. However, the underlying mechanisms are largely unknown. In this study, to clarify the role of autophagy in β-CG-induced cytotoxicity, we investigated the effects of β-CG on cell viability and autophagy activity in porcine enterocytes (IPEC-1 cells). The results indicated that the cell viability was decreased with the increasing levels of β-CG. β-CG treatment enhanced the eGFP-LC3 puncta per cells and LC3-II/LC3-I, and the latter was further increased in IPEC-1 cells cultured with bafilomycin A1. We conclude that β-CG enhances autophagy activity in enterocytes.

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Fig. 1
Fig. 2

Abbreviations

β-CG:

β-Conglycinin

Baf A1:

Bafilomycin A1

IPEC-1:

Intestinal porcine epithelial cell 1

LC3:

Microtubule-associated protein light chain 3

mTOR:

Mammalian target of rapamycin

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Acknowledgments

This research was jointly supported by National Natural Science Foundation of China (Nos. 31372319, 31572416, 31402084), the Hubei Provincial Key Project for Scientific and Technical Innovation (2014ABA022), Natural Science Foundation of Hubei Province (Nos. 2013CFA097, 2012FFB04805), the Hubei Hundred Talent Program, Agriculture and Food Research Initiative Competitive Grants of (2014-67015-21770) of the USDA National Institute of Food and Agriculture, and Texas AgriLife Research (H-82000).

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Authors and Affiliations

  1. Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety, Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, 430023, China

    Dan Yi, Yongqing Hou, Huimin Mei, Lei Wang, Chien-An Andy Hu & Guoyao Wu

  2. Department of Biochemistry and Molecular Biology, University of New Mexico School of Medicine, Albuquerque, NM, 87131, USA

    Chien-An Andy Hu

  3. Department of Animal Science, Texas A&M University, College Station, TX, 77843, USA

    Guoyao Wu

Authors
  1. Dan Yi
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  2. Yongqing Hou
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  3. Huimin Mei
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  4. Lei Wang
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  5. Chien-An Andy Hu
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  6. Guoyao Wu
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Corresponding author

Correspondence to Yongqing Hou.

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The authors declare that they have no conflict of interest.

Ethics statement

This study involved an established porcine intestinal cell line and did not require the approval of animal use.

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This study did not involve informed consent.

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Yi, D., Hou, Y., Mei, H. et al. β-Conglycinin enhances autophagy in porcine enterocytes. Amino Acids 49, 203–207 (2017). https://doi.org/10.1007/s00726-016-2352-7

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