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Supplementation with l-glutamine prevents tumor growth and cancer-induced cachexia as well as restores cell proliferation of intestinal mucosa of Walker-256 tumor-bearing rats

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Abstract

This study aimed to evaluate the intestinal mucosa of the duodenum and jejunum of Walker-256 tumor-bearing rats supplemented with l-glutamine. Thirty-two male 50-day-old Wistar rats (Rattus norvegicus) were randomly divided into four groups: control (C), control supplemented with 2 % l-glutamine (GC), Walker-256 tumor (WT), and Walker-256 tumor supplemented with 2 % l-glutamine (TWG). Walker-256 tumor was induced by inoculation viable tumor cells in the right rear flank. After 10 days, celiotomy was performed and duodenal and jejunal tissues were removed and processed. We evaluated the cachexia index, proliferation index, villus height, crypt depth, total height of the intestinal wall, and number of goblet cells by the technique of periodic acid-Schiff (PAS). Induction of Walker-256 tumor promoted a reduction of metaphase index in the TW group animals, which was accompanied by a reduction in the villous height and crypt depths, resulting in atrophy of the intestinal wall as well as increased PAS-positive goblet cells. Supplementation with l-glutamine reduced the tumor growth and inhibited the development of the cachectic syndrome in animals of the TWG group. Furthermore, amino acid supplementation promoted beneficial effects on the intestinal mucosa in the TWG animals through restoration of the number of PAS-positive goblet cells. Therefore, supplementation with 2 % l-glutamine exhibited a promising role in the prevention of tumor growth and cancer-associated cachexia as well as restoring the intestinal mucosa in the duodenum and jejunum of Walker-256 tumor-bearing rats.

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Authors and Affiliations

  1. Department of Pharmacy, Universidade Estadual de Maringá, Maringá, PR, Brazil

    Heber Amilcar Martins, Camila Caviquioli Sehaber, Catchia Hermes-Uliana, Geraldo Emílio Vicentini, Gleison Daion Piovezana Bossolani, Mariana Machado Lima & Roberto Barbosa Bazotte

  2. Department of Pharmacy, Centro Universitário Cesumar, Maringá, PR, Brazil

    Fernando Augusto Mariani & Laraine Almeida Jussani

  3. Department of General Pathology, Universidade Estadual de Londrina, Londrina, PR, Brazil

    Flavia Alessandra Guarnier

  4. Department of Morphological Sciences, Universidade Estadual de Maringá, Avenida Colombo, No. 5790 Bloco H-79, Maringá, PR, CEP 87020-900, Brazil

    Jacqueline Nelisis Zanoni

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  1. Heber Amilcar Martins
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  2. Camila Caviquioli Sehaber
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  3. Catchia Hermes-Uliana
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  5. Flavia Alessandra Guarnier
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  8. Laraine Almeida Jussani
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  9. Mariana Machado Lima
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  11. Jacqueline Nelisis Zanoni
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Correspondence to Jacqueline Nelisis Zanoni.

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Fundação Araucária/266/2014.

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The authors declare that they have no conflict of interest.

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Handling Editor: G. J. Peters.

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Martins, H.A., Sehaber, C.C., Hermes-Uliana, C. et al. Supplementation with l-glutamine prevents tumor growth and cancer-induced cachexia as well as restores cell proliferation of intestinal mucosa of Walker-256 tumor-bearing rats. Amino Acids 48, 2773–2784 (2016). https://doi.org/10.1007/s00726-016-2313-1

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  • DOI: https://doi.org/10.1007/s00726-016-2313-1

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