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Identification and characterization of novel anticoagulant peptide with thrombolytic effect and nutrient oligopeptides with high branched chain amino acid from Whitmania pigra protein

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Abstract

Natural and nutrient substances for cardiovascular disease are promising and capture researchers’ minds. Two kinds of novel bioactive peptides (high Fischer’s ratio oligopeptides and anticoagulant peptides) were obtained from Whitmania pigra protein via enzymatic hydrolysis. An oligopeptide (MW<874.0 Da) named as HF2 was obtained via chromatography purification procedures with a high Fischer’s ratio of 31.92 ± 1.36 and low phenylalanine + tyrosine content of 0.98 ± 0.04 %. Another peptide (WA3-1), prepared by alcalase AF 2.4 L-catalyzed hydrolysis and then purified by DEAE Sepharose FF, gel Sephadex G-15 chromatography, exhibited high anticoagulant activity with prolonging significantly plasma clotting time on activated partial thromboplastin time, prothrombin time, thrombin time (p < 0.01) and powerful thrombolytic activity. Amino acid composition and MALDI-TOF/TOF MS analysis showed that WA3-1 contained 11 amino acids (MW: 1422.0 Da) with the sequence as NH2–His-Asp-Phe-Leu-Asn-Asn-Lys-Leu-Glu-Tyr-Glu–COOH. Abundant negatively charged amino acids in C-terminal, as well as the special residue Lys contribute to its anticoagulant capacity. This research provided a novel natural candidate for the manufacture of nutrient oligopeptides with high branched chain amino acid, and anticoagulant thrombolytic agent in pharmaceutical industry with helping prevent from thrombosis and related cardiovascular diseases.

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Acknowledgments

The authors are grateful to Program for New Century Excellent Talents in University (NCET-12-0192), and Scientific Research Foundation for Young Teachers of Sichuan University (2016SCU11035) for the financial supports.

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Authors and Affiliations

  1. College of Light Industry, Textile and Food Engineering, Sichuan University, Chengdu, 610065, China

    Yao Ren

  2. College of Light Industry and Food Sciences, South China University of Technology, Wushan Road 381, Guangzhou, 510641, China

    Yijing Yang, Wenjia Wu, Mengmeng Zhang, Hui Wu & Xiaofeng Li

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  1. Yao Ren
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  2. Yijing Yang
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  3. Wenjia Wu
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  4. Mengmeng Zhang
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  5. Hui Wu
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  6. Xiaofeng Li
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Correspondence to Hui Wu or Xiaofeng Li.

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Handling Editor: M. S. Palma.

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Ren, Y., Yang, Y., Wu, W. et al. Identification and characterization of novel anticoagulant peptide with thrombolytic effect and nutrient oligopeptides with high branched chain amino acid from Whitmania pigra protein. Amino Acids 48, 2657–2670 (2016). https://doi.org/10.1007/s00726-016-2299-8

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  • DOI: https://doi.org/10.1007/s00726-016-2299-8

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