Rapid quantitative method for the detection of phenylalanine and tyrosine in human plasma using pillar array columns and gradient elution
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This study reports a fast and quantitative determination method for phenylalanine (Phe) and tyrosine (Tyr) in human plasma using on-chip pressure-driven liquid chromatography. A pillar array column with low-dispersion turns and a gradient elution system was used. The separation of fluorescent derivatives of Phe, Tyr, and other hydrophobic amino acids was successfully performed within 140 s. Under the optimized conditions, Phe and Tyr in human plasma were quantified. The developed method is promising for rapid diagnosis in the clinical field.
KeywordsMicrofluidics 4-Fluoro-7-nitro-2,1,3-benzoxadiazole Fluorescence Quantitative determination Amino acids
This research is supported by the Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C) grant number 26460033 and the Center of Innovation Program from Japan Science and Technology Agency, JST.
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Conflict of interest
The authors declare that they have no conflict of interest.
This article does not contain any studies with human participants performed by any of the authors.
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