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Amino Acids

, Volume 48, Issue 7, pp 1731–1735 | Cite as

Rapid quantitative method for the detection of phenylalanine and tyrosine in human plasma using pillar array columns and gradient elution

  • Yanting Song
  • Katsuya Takatsuki
  • Tetsushi Sekiguchi
  • Takashi Funatsu
  • Shuichi Shoji
  • Makoto TsunodaEmail author
Short Communication

Abstract

This study reports a fast and quantitative determination method for phenylalanine (Phe) and tyrosine (Tyr) in human plasma using on-chip pressure-driven liquid chromatography. A pillar array column with low-dispersion turns and a gradient elution system was used. The separation of fluorescent derivatives of Phe, Tyr, and other hydrophobic amino acids was successfully performed within 140 s. Under the optimized conditions, Phe and Tyr in human plasma were quantified. The developed method is promising for rapid diagnosis in the clinical field.

Keywords

Microfluidics 4-Fluoro-7-nitro-2,1,3-benzoxadiazole Fluorescence Quantitative determination Amino acids 

Notes

Acknowledgments

This research is supported by the Japan Society for the Promotion of Science, Grant-in-Aid for Scientific Research (C) grant number 26460033 and the Center of Innovation Program from Japan Science and Technology Agency, JST.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

This article does not contain any studies with human participants performed by any of the authors.

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Copyright information

© Springer-Verlag Wien 2016

Authors and Affiliations

  • Yanting Song
    • 1
    • 2
  • Katsuya Takatsuki
    • 3
  • Tetsushi Sekiguchi
    • 3
  • Takashi Funatsu
    • 1
  • Shuichi Shoji
    • 3
  • Makoto Tsunoda
    • 1
    Email author
  1. 1.Graduate School of Pharmaceutical SciencesUniversity of TokyoTokyoJapan
  2. 2.Key Laboratory of Tropic Biological Resources, Minister of Education, College of Marine ScienceHainan UniversityHaikouChina
  3. 3.Major in Nano-Science and Nano-EngineeringWaseda UniversityTokyoJapan

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