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Proline hydration at low temperatures: its role in the protection of cell from freeze-induced stress

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Abstract

The natural amino acid l-α-proline (Pro) is a compatible osmolyte which accumulates in the cell cytoplasm to protect structure and function of various proteins and enzymes under abiotic stress, like for instance, freezing. It is assumed that the interactions of Pro with intracellular water play an important role in the protection mechanism. However, until now the details of these interactions are far from being fully understood. We present results of a theoretical study of the hydration of Pro zwitterion (Pro-ZW) in water in the temperature range of 298–248 K. The data were obtained by the integral equation method in the framework of the 1D- and 3D-RISM approaches. The structural data were analyzed in terms of radial and spatial distribution functions. The observed features of Pro-ZW hydration are discussed from the position of the biological role of Pro as a cryoprotectant. In particular, it was found that under cooling conditions this protectant is able to bind a significant amount of water molecules and, thus, is helping to keep water inside the cell.

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Acknowledgments

This work was partly supported by the Russian Foundation for Basic Research with Grant 15-43-03004-r_centre_a.

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  1. G.A. Krestov Institute of Solution Chemistry, Russian Academy of Sciences, Akademicheskaya st. 1, Ivanovo, 153045, Russia

    Marina V. Fedotova & Olga A. Dmitrieva

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  1. Marina V. Fedotova
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  2. Olga A. Dmitrieva
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Correspondence to Marina V. Fedotova.

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Fedotova, M.V., Dmitrieva, O.A. Proline hydration at low temperatures: its role in the protection of cell from freeze-induced stress. Amino Acids 48, 1685–1694 (2016). https://doi.org/10.1007/s00726-016-2232-1

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