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Proteome-derived peptide library for the elucidation of the cleavage specificity of HF3, a snake venom metalloproteinase

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Abstract

The Proteomic Identification of Cleavage Sites (PICS) approach was employed for profiling the substrate specificity of HF3, a hemorrhagic snake venom metalloproteinase (SVMP) from Bothrops jararaca. A tryptic peptide library from human plasma was subject to HF3 cleavage and amino acid occurrence for P6 to P6′ sites was mapped. 71 cleavage sites were detected and revealed a clear preference for leucine at P1′ position, followed by hydrophobic residues in P2′. PICS confirmed existing data on prime site specificity of SVMPs.

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Acknowledgments

This work was supported by grants from Fundação de Amparo à Pesquisa do Estado de São Paulo (11/16623-1; 13/07467-1), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (1214/2011) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (304103/2012-8).

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Authors and Affiliations

  1. Laboratório Especial de Toxinologia Aplicada, Center of Toxins, Immune-response and Cell Signaling, CeTICS, Instituto Butantan, Av. Vital Brasil 1500, São Paulo, 05503-000, Brazil

    Luciana Bertholim, André Zelanis, Ana K. Oliveira & Solange M. T. Serrano

  2. Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, Brazil

    Luciana Bertholim & Ana K. Oliveira

  3. Departamento de Ciência e Tecnologia, Universidade Federal de São Paulo, São José dos Campos, SP, Brazil

    André Zelanis

Authors
  1. Luciana Bertholim
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  2. André Zelanis
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  3. Ana K. Oliveira
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  4. Solange M. T. Serrano
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Corresponding author

Correspondence to Solange M. T. Serrano.

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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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Informed consent was obtained from all individual participants included in the study.

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Handling Editor: M. S. Palma.

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Bertholim, L., Zelanis, A., Oliveira, A.K. et al. Proteome-derived peptide library for the elucidation of the cleavage specificity of HF3, a snake venom metalloproteinase. Amino Acids 48, 1331–1335 (2016). https://doi.org/10.1007/s00726-016-2218-z

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  • DOI: https://doi.org/10.1007/s00726-016-2218-z

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