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Hypotensive effect of S-adenosyl-l-methionine in hypertensive rats is reduced by autonomic ganglia and KATP channel blockers

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Abstract

S-adenosyl-l-methionine (SAM) is an amino acid involved in a number of physiological processes in the nervous system. Some evidence suggests a therapeutic potential of SAM in hypertension. In this study we investigated the effect of intracerebroventricular (ICV) infusions of SAM on arterial blood pressure in rats. Mean arterial blood pressure (MABP) and heart rate (HR) were measured at baseline and during ICV infusion of either SAM or vehicle (aCSF; controls) in conscious, male normotensive Wistar Kyoto rats (WKY) and Spontaneously Hypertensive Rats (SHR). MABP and HR were not affected by the vehicle. WKY rats infused with SAM (10 μM, 100 μM and 1 mM) showed a biphasic hemodynamic response i.e., mild hypotension and bradycardia followed by a significant increase in MABP and HR. On the contrary, SHR infused with SAM showed a dose-dependent hypotensive response. In separate series of experiments, pretreatment with hexamethonium, a ganglionic blocker as well as pretreatment with glibenclamide, a KATP channel blocker reduced the hemodynamic effects of SAM. SAM may affect the nervous control of arterial blood pressure via the autonomic nervous system and KATP channel-dependent mechanisms.

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Acknowledgments

This study was funded by the National Science Center Grant No. 2011/01/N/NZ4/03682 and Medical University of Warsaw.

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Authors and Affiliations

  1. Department of Experimental Physiology and Pathophysiology, Laboratory of Centre for Preclinical Research, The Medical University of Warsaw, Warsaw, Poland

    Mariusz Sikora, Kinga Pham & Marcin Ufnal

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  1. Mariusz Sikora
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  2. Kinga Pham
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  3. Marcin Ufnal
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Correspondence to Mariusz Sikora.

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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted.

This article does not contain any studies with human participants performed by any of the authors.

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Handling Editor: H. Jakubowski.

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Sikora, M., Pham, K. & Ufnal, M. Hypotensive effect of S-adenosyl-l-methionine in hypertensive rats is reduced by autonomic ganglia and KATP channel blockers. Amino Acids 48, 1581–1590 (2016). https://doi.org/10.1007/s00726-016-2213-4

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