Abstract
This review focuses on the various approaches to covalently attach a chromophore to a biomolecule of interest in site-specific manner. Novel methods like inverse electron-demand Diels–Alder reaction, Pictet-Spengler ligation and enzyme tags like SNAP and Halo-tags are critically discussed and compared to established techniques like copper-free click reaction and native chemical ligation. Selected examples in which the tags have been exploited for in vitro or in vivo imaging are reviewed and evaluated.
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Freidel, C., Kaloyanova, S. & Peneva, K. Chemical tags for site-specific fluorescent labeling of biomolecules. Amino Acids 48, 1357–1372 (2016). https://doi.org/10.1007/s00726-016-2204-5
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DOI: https://doi.org/10.1007/s00726-016-2204-5