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Amino Acids

, Volume 47, Issue 7, pp 1283–1299 | Cite as

Synthetic strategies for polypeptides and proteins by chemical ligation

  • Ming Chen
  • Pascal Heimer
  • Diana ImhofEmail author
Review Article

Abstract

This review focuses on chemical ligation methods for the preparation of oligopeptides and proteins. Chemical ligation is a practical and convenient methodology in peptide and protein synthesis. Longer peptides and proteins can be obtained with high yield in aqueous buffer solutions by coupling unprotected peptide segments even without activation by enzymes or further chemical agents. Several methods and protocols were developed in the past. The potential of the most important approaches of the thioester- and imine-ligation techniques is demonstrated by a broad spectrum of applications. In addition, special features and protocols such as the template-directed ligation, ligation with novel additives or solvent media, microwave-assisted ligation, and the achievements obtained with those are also highlighted herein.

Keywords

Chemical ligation Native chemical ligation Thioester Chemoselective synthesis Protein synthesis 

Notes

Acknowledgments

We thank the DAAD and the Chinese Scholarship Council for financial support.

Conflict of interest

The authors declare that they have no conflict of interest.

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© Springer-Verlag Wien 2015

Authors and Affiliations

  1. 1.Pharmaceutical Chemistry I, Institute of PharmacyUniversity of BonnBonnGermany

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