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Amino Acids

, Volume 47, Issue 9, pp 1847–1852 | Cite as

Plasma and tissue homoarginine concentrations in healthy and obese humans

  • Marcus MayEmail author
  • Arslan Arinc Kayacelebi
  • Sandor Batkai
  • Jens Jordan
  • Dimitrios Tsikas
  • Stefan Engeli
Original Article
Part of the following topical collections:
  1. Homoarginine, Arginine and Relatives

Abstract

Increased cardiovascular risk associated with obesity cannot be fully explained by traditional risk markers. We therefore assessed plasma and interstitial concentrations of the novel cardiovascular risk biomarker homoarginine (hArg) in 18 individuals without signs of cardiovascular disease, including 4 morbidly obese subjects before and after bariatric surgery and subsequent weight reduction of 36 ± 7 kg. hArg concentrations were greater in skeletal muscle compared with adipose tissue. Plasma and tissue hArg concentrations did not correlate with BMI. Adipose tissue interstitial hArg concentrations were not affected by obesity, an oral glucose load, or dramatic weight loss. In conclusion, obesity seems not to have a major effect on hArg homeostasis, and hArg may not explain the added cardiovascular risk associated with obesity. Yet, given the small sample size of the study, the significance of hArg in obesity should be investigated in a larger population.

Keywords

Adipose tissue Skeletal muscle Biomarker Insulin resistance L-Homoarginine Microdialysis Obesity 

Abbreviations

ADMA

N G,N G-Dimethyl-l-arginine

BMI

Body mass index

hArg

L-Homoarginine

HOMA-IR

Homeostasis model assessment-insulin resistance (index)

MD

Microdialysis

NO

Nitric oxide

NOS

Nitric oxide synthase

SRM

Selected-reaction monitoring

Notes

Acknowledgments

The laboratory assistance of Bibiana Beckmann, Anja Mitschke and Maria-Theresia Suchy is gratefully acknowledged. We thank Frank-Mathias Gutzki for performing GC–MS/MS analyses of homoarginine and ADMA in plasma and microdialysate samples.

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag Wien 2015

Authors and Affiliations

  • Marcus May
    • 1
    Email author
  • Arslan Arinc Kayacelebi
    • 2
  • Sandor Batkai
    • 3
  • Jens Jordan
    • 1
  • Dimitrios Tsikas
    • 2
  • Stefan Engeli
    • 1
  1. 1.Institute of Clinical PharmacologyHannover Medical SchoolHannoverGermany
  2. 2.Centre of Pharmacology and ToxicologyHannover Medical SchoolHannoverGermany
  3. 3.Institute of Molecular and Translational Therapeutic StrategiesHannover Medical SchoolHannoverGermany

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