Plasma and tissue homoarginine concentrations in healthy and obese humans
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Increased cardiovascular risk associated with obesity cannot be fully explained by traditional risk markers. We therefore assessed plasma and interstitial concentrations of the novel cardiovascular risk biomarker homoarginine (hArg) in 18 individuals without signs of cardiovascular disease, including 4 morbidly obese subjects before and after bariatric surgery and subsequent weight reduction of 36 ± 7 kg. hArg concentrations were greater in skeletal muscle compared with adipose tissue. Plasma and tissue hArg concentrations did not correlate with BMI. Adipose tissue interstitial hArg concentrations were not affected by obesity, an oral glucose load, or dramatic weight loss. In conclusion, obesity seems not to have a major effect on hArg homeostasis, and hArg may not explain the added cardiovascular risk associated with obesity. Yet, given the small sample size of the study, the significance of hArg in obesity should be investigated in a larger population.
KeywordsAdipose tissue Skeletal muscle Biomarker Insulin resistance L-Homoarginine Microdialysis Obesity
N G,N G-Dimethyl-l-arginine
Body mass index
Homeostasis model assessment-insulin resistance (index)
Nitric oxide synthase
The laboratory assistance of Bibiana Beckmann, Anja Mitschke and Maria-Theresia Suchy is gratefully acknowledged. We thank Frank-Mathias Gutzki for performing GC–MS/MS analyses of homoarginine and ADMA in plasma and microdialysate samples.
Conflict of interest
The authors declare that they have no conflict of interest.
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