Abstract
Infections by enterotoxigenic Escherichia coli (ETEC) result in large economic losses to the swine industry worldwide. Dietary supplementation with amino acids has been considered as a potential mechanism to improve host defenses against infection. The goal of this study was to determine whether methionine deprivation alters ETEC interactions with porcine intestinal epithelial cells. IPEC-1 cells were cultured in media with or without l-methionine. Methionine deprivation resulted in enhanced ETEC adhesion and increased both the cytotoxicity and apoptotic responses of IPEC-1 cells infected with ETEC. Methionine deprivation inhibited IPEC-1 cell autophagic responses, suggesting that the increased cytotoxicity of ETEC to methionine-deprived IPEC-1 cells might be due to defects in autophagy.
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Acknowledgments
This work was supported by the National Natural Science Foundation of China (No. 31110103909, 31272217, 31101729, and 31201813), National Science and Technology Support Program Funding (2012BAD39B03), Nanjing Branch Academy of Chinese Academy of Science and Jiangxi Province Cooperation Project, Hunan Project (2013RS4065 and 2012GK4066) and Guandong project (2012B091100210).
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Tang, Y., Tan, B., Xiong, X. et al. Methionine deficiency reduces autophagy and accelerates death in intestinal epithelial cells infected with enterotoxigenic Escherichia coli . Amino Acids 47, 2199–2204 (2015). https://doi.org/10.1007/s00726-014-1781-4
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DOI: https://doi.org/10.1007/s00726-014-1781-4