Amino Acids

, Volume 46, Issue 9, pp 2165–2175 | Cite as

Metabolic engineering Corynebacterium glutamicum for the l-lysine production by increasing the flux into l-lysine biosynthetic pathway

  • Jianzhong Xu
  • Mei Han
  • Junlan Zhang
  • Yanfeng Guo
  • Weiguo ZhangEmail author
Original Article


The experiments presented here were based on the conclusions of our previous results. In order to avoid introduction of expression plasmid and to balance the NADH/NAD ratio, the NADH biosynthetic enzyme, i.e., NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (GADPH), was replaced by NADP-dependent GADPH, which was used to biosynthesize NADPH rather than NADH. The results indicated that the NADH/NAD ratio significantly decreased, and glucose consumption and l-lysine production drastically improved. Moreover, increasing the flux through l-lysine biosynthetic pathway and disruption of ilvN and hom, which involve in the branched amino acid and l-methionine biosynthesis, further improved l-lysine production by Corynebacterium glutamicum. Compared to the original strain C. glutamicum Lys5, the l-lysine production and glucose conversion efficiency (α) were enhanced to 81.0 ± 6.59 mM and 36.45 % by the resulting strain C. glutamicum Lys5-8 in shake flask. In addition, the by-products (i.e., l-threonine, l-methionine and l-valine) were significantly decreased as results of genetic modification in homoserine dehydrogenase (HSD) and acetohydroxyacid synthase (AHAS). In fed-batch fermentation, C. glutamicum Lys5-8 began to produce l-lysine at post-exponential growth phase and continuously increased over 36 h to a final titer of 896 ± 33.41 mM. The l-lysine productivity was 2.73 g l−1 h−1 and the α was 47.06 % after 48 h. However, the attenuation of MurE was not beneficial to increase the l-lysine production because of decreasing the cell growth. Based on the above-mentioned results, we get the following conclusions: cofactor NADPH, precursor, the flux through l-lysine biosynthetic pathway and DCW are beneficial to improve l-lysine production in C. glutamicum.


Corynebacterium glutamicum NAD-dependent GADPH NADP-dependent GADPH l-Lysine biosynthetic pathway By-products minimization 



This work was financially supported by the Program of Chinese 863 National High-Tech Research and Development Plan Project (No. 2008AA02Z212) and by the Priority Academic Program Development of Jiangsu Higher Education Institutions.

Conflict of interest

The authors declare no commercial or financial conflict of interest.

Supplementary material

726_2014_1768_MOESM1_ESM.pdf (227 kb)
Supplementary material 1 (PDF 153 kb)


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Copyright information

© Springer-Verlag Wien 2014

Authors and Affiliations

  • Jianzhong Xu
    • 1
  • Mei Han
    • 1
    • 2
  • Junlan Zhang
    • 3
  • Yanfeng Guo
    • 1
  • Weiguo Zhang
    • 1
    Email author
  1. 1.The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of BiotechnologyJiangnan UniversityWuXiPeople’s Republic of China
  2. 2.State Key Laboratory of Dairy BiotechnologyShangHaiPeople’s Republic of China
  3. 3.OriGene Biotechnology Co., Ltd.WuXiPeople’s Republic of China

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