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Amino Acids

, Volume 46, Issue 9, pp 2219–2229 | Cite as

Metabolomic analysis of amino acid and energy metabolism in rats supplemented with chlorogenic acid

  • Zheng Ruan
  • Yuhui Yang
  • Yan Zhou
  • Yanmei Wen
  • Sheng Ding
  • Gang Liu
  • Xin Wu
  • Peng LiaoEmail author
  • Zeyuan Deng
  • Houssein Assaad
  • Guoyao Wu
  • Yulong YinEmail author
Original Article

Abstract

This study was conducted to investigate effects of chlorogenic acid (CGA) supplementation on serum and hepatic metabolomes in rats. Rats received daily intragastric administration of either CGA (60 mg/kg body weight) or distilled water (control) for 4 weeks. Growth performance, serum biochemical profiles, and hepatic morphology were measured. Additionally, serum and liver tissue extracts were analyzed for metabolomes by high-resolution 1H nuclear magnetic resonance-based metabolomics and multivariate statistics. CGA did not affect rat growth performance, serum biochemical profiles, or hepatic morphology. However, supplementation with CGA decreased serum concentrations of lactate, pyruvate, succinate, citrate, β-hydroxybutyrate and acetoacetate, while increasing serum concentrations of glycine and hepatic concentrations of glutathione. These results suggest that CGA supplementation results in perturbation of energy and amino acid metabolism in rats. We suggest that glycine and glutathione in serum may be useful biomarkers for biological properties of CGA on nitrogen metabolism in vivo.

Keywords

Chlorogenic acid Amino acids Metabolism Nuclear magnetic resonance spectroscopy 

Abbreviations

CGA

Chlorogenic acid

PC

Principal components

PCA

Principal component analysis

NMR

Nuclear magnetic resonance

Notes

Acknowledgments

This research was financially supported by National Natural Science Foundation of China (Grant No. 31001014), the Research Foundation (SKLF-TS-201108 and SKLF-TS-200817) and the Open Project Program (SKLF-KF-201005 and SKLF-KF-201216) of State Key Laboratory of Food Science and Technology at Nanchang University, and Texas A&M AgriLife Research H82000. H. Assaad was supported by a postdoctoral training grant (R25T-CA090301) from the National Cancer Institute.

Conflict of interest

The authors declare that they have no conflict of interests.

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Copyright information

© Springer-Verlag Wien 2014

Authors and Affiliations

  • Zheng Ruan
    • 1
  • Yuhui Yang
    • 1
  • Yan Zhou
    • 1
  • Yanmei Wen
    • 1
  • Sheng Ding
    • 2
  • Gang Liu
    • 3
  • Xin Wu
    • 3
  • Peng Liao
    • 3
    • 4
    Email author
  • Zeyuan Deng
    • 1
  • Houssein Assaad
    • 5
  • Guoyao Wu
    • 5
  • Yulong Yin
    • 1
    • 3
    Email author
  1. 1.State Key Laboratory of Food Science and Technology, College of Life Science and Food EngineeringNanchang UniversityNanchangChina
  2. 2.Institute of Nutrition and Food SafetyCenter for Disease Control and Prevention of Jiangxi ProvinceNanchangChina
  3. 3.Hunan Engineering and Research Center of Animal and Poultry Science, Institute of Subtropical AgricultureChinese Academy of SciencesChangshaChina
  4. 4.Hunan New Wellful Co., LTDChangshaChina
  5. 5.Department of Animal ScienceTexas A&M UniversityCollege StationUSA

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