Abstract
Differential anti-prion activity of polylysine enantiomers was studied. Based on our recent discovery that poly-l-lysine (PLK) is a potent anti-prion agent, we investigated suppression of prions in cultured cells using poly-d-lysine (PDK). The results showed that PDK was more efficacious than PLK to inhibit prions. Protein misfolding cyclic amplification assay demonstrated improved efficacy of PDK in inhibiting plasminogen-mediated prion propagation, corresponding to the enantio-preference of PDK observed in cultured cells. Furthermore, our study demonstrated that polylysines formed a complex with plasminogen. These results propose to hypothesize a plausible mechanism that elicits prion inhibition by polylysine enantiomers.
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The authors thank P. Thomason for editorial and H. Lee for technical assistance.
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K. S. Jackson and J. Yeom contributed equally to this work.
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Jackson, K.S., Yeom, J., Han, Y. et al. Preference toward a polylysine enantiomer in inhibiting prions. Amino Acids 44, 993–1000 (2013). https://doi.org/10.1007/s00726-012-1430-8
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DOI: https://doi.org/10.1007/s00726-012-1430-8