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Analysing signalling networks by mass spectrometry

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Abstract

Sequence analysis of the human genome and the association of genetic aberrations with diseases have provided a rough framework whereby the impact of individual genotypes can be assessed. To fully understand the effect of individual and co-occurring genetic aberrations, as well as their individual and collected contribution to the development of diseases, it is critical to analyse the matching proteome and to determine how the organisation, expression level and function of protein networks are affected. Sensitive mass spectrometric platforms in combination with innovative workflows allow qualitative and quantitative analyses of the cellular as well as the extracellular proteome. Importantly, in addition to specifically identifying the content of the proteome, several aspects of the proteomic organisation can be analysed including protein complexes, protein modifications, enzymatic activities and subcellular/organelle localisation. Together, these measurements will provide novel insight into the biological effect of disease-causing mutations ultimately coupling genotype and phenotype.

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Acknowledgments

The authors would like to thank members of the cell communication team for valuable input and discussions. The research in the cell communication team is generously supported by BBSRC and CR-UK career establishment award.

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  1. Division of Cancer Biology, Cell Communication Team, The Institute of Cancer Research, London, SW3 6JB, UK

    Claus Jørgensen & Marie Locard-Paulet

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Correspondence to Claus Jørgensen.

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Jørgensen, C., Locard-Paulet, M. Analysing signalling networks by mass spectrometry. Amino Acids 43, 1061–1074 (2012). https://doi.org/10.1007/s00726-012-1293-z

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