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Development and application of site-specific proteomic approach for study protein S-nitrosylation

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Abstract

Protein S-nitrosylation is the covalent redox-related modification of cysteine sulfhydryl groups with nitric oxide, creating a regulatory impact similar to phosphorylation. Recent studies have reported a growing number of proteins to be S-nitrosylated in vivo resulting in altered functions. These studies support S-nitrosylation as a critical regulatory mechanism, fine-tuning protein activities within diverse cellular processes and biochemical pathways. In addition, S-nitrosylation appears to have key roles in the etiology of a broad range of human diseases. In this review, we discuss recent advances in proteomic approaches for the enrichment, identification, and quantitation of cysteine S-nitrosylated proteins and peptides. These advances have provided analytical tools with the power to interpret the impact of S-nitrosylation at the system level, providing a new platform for drug discovery and the identification of diagnostic markers for human diseases.

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Acknowledgments

We thank Dr. Lawrence M. Schopfer and Stites M. Kirsten for editing this manuscript. This work was supported by the Department of Pathology and Microbiology at the University of Nebraska Medical Center (UNMC), NEHHS LB606 to S.J.D., Nebraska Research Initiative to S.J.D. and J.E.T., M.L. is supported by a fellowship from the College of Medicine at UNMC.

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Authors and Affiliations

  1. Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE, 68198, USA

    Miao Liu

  2. Laboratory of Transplantation Immunology, Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE, 68198, USA

    James E. Talmadge

  3. Department of Pathology and Microbiology, Mass Spectrometry and Proteomics Core Facility, University of Nebraska Medical Center, Omaha, NE, 68198, USA

    Shi-Jian Ding

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  1. Miao Liu
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  2. James E. Talmadge
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Correspondence to Shi-Jian Ding.

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Liu, M., Talmadge, J.E. & Ding, SJ. Development and application of site-specific proteomic approach for study protein S-nitrosylation. Amino Acids 42, 1541–1551 (2012). https://doi.org/10.1007/s00726-012-1279-x

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  • DOI: https://doi.org/10.1007/s00726-012-1279-x

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