Protective immunity provided by a new modified SERA protein peptide: its immunogenetic characteristics and correlation with 3D structure
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The serine repeat antigen (SERA) protein is a leading candidate molecule for inclusion as a component in a multi-antigen, multi-stage, minimal subunit-based, chemically synthesised anti-malarial vaccine. Peptides having high red blood cell binding affinity (known as HABPs) have been identified in this protein. The 6733 HABP was located in the C-terminal portion of the 47-kDa fragment while HABP 6754 was located in the C-terminal region of the 56-kDa fragment. These conserved HABPs failed to induce an immune response. Critical red blood cell binding residues and/or their neighbours (assessed by glycine-analogue scanning) were replaced by others having the same mass, volume and surface but different polarity, rendering some of them highly immunogenic when assessed by antibody production against the parasite or its proteins and protection-inducers against experimental challenge with a highly infectious Aotus monkey-adapted Plasmodium falciparum strain. This manuscript presents some modified HABPs as vaccine candidate components for enriching our tailor-made anti-malarial vaccine repertoire, as well as their 3D structure obtained by 1H-NMR displaying a short-structured region, differently from the native ones having random structures.
KeywordsSERA 5 NMR Structure Malaria vaccine
We would like to thank Jason Garry for helping in the translation of the manuscript, Sylvain Meguellatni for helping with technical support regarding Bruker equipment and Martha Forero and Yolanda Silva for their continued help with the immunochemical assays.
Conflict of interest
The authors declare that they have no conflict of interest.
- Alba MP, Salazar LM, Puentes A, Pinto M, Torres E, Patarroyo ME (2003) 6746 SERA peptide analogues immunogenicity and protective efficacy against malaria is associated with short alpha helix formation: malaria protection associated with peptides alpha helix shortening. Peptides 24:999–1006PubMedCrossRefGoogle Scholar
- Bax A, Davis DG (1985) MLEV-17-based two-dimensional homonuclear magnetization transfer spectroscopy. J Magn Resonance 65:355–360Google Scholar
- Bermudez A, Reyes C, Guzman F, Vanegas M, Rosas J, Amador R, Rodriguez R, Patarroyo MA, Patarroyo ME (2007) Synthetic vaccine update: applying lessons learned from recent SPf66 malarial vaccine physicochemical, structural and immunological characterization. Vaccine 25:4487–4501PubMedCrossRefGoogle Scholar