Amino Acids

, Volume 40, Issue 4, pp 1107–1113 | Cite as

Kinetics of amino acid production from bean dregs by hydrolysis in sub-critical water

  • Guangyong Zhu
  • Xian ZhuEmail author
  • Qi Fan
  • Xueliang Wan
Original Article


Amino acids play an important physiological role in all life-forms and can be recovered from bean dregs waste using sub-critical water hydrolysis. This work deals with the hydrolysis kinetics of bean dregs. Kinetics was conducted in a temperature range of 200–240°C using a 300-ml stainless steel batch reactor. Since the reaction kinetics in sub-critical water is very complicated, a simplified kinetic model to describe the hydrolysis of bean dregs is proposed: a single consecutive reaction. The differential equations resulting from the model were fit to experimental data to obtain kinetic rate constants. By means of the Arrhenius plot, the activation energy as well as the pre-exponential factor was determined. A good agreement between the simplified model and the experimental data was obtained. The kinetic parameters provided useful information for understanding the hydrolysis reaction of bean dregs. The experimental results show that the best hydrolysis technology is: reaction temperature 200°C, reaction time 20 min. Under this condition, the total amino acid yield reaches 52.9%. Based on the results, this method could become an efficient method for bean dregs liquefaction, producing valuable amino acid.


Kinetics Hydrolysis Bean dregs Sub-critical water Amino acids 



The authors thank the National Natural Science Fund of China (50578091) and Shanghai Leading Academic Disciplines (S30109) for financial support.


  1. Alenezi R, Leeke GA, Santos RCD, Khan AR (2009) Hydrolysis kinetics of sunflower oil under subcritical water conditions. Chem Eng Res Des 87:867–873CrossRefGoogle Scholar
  2. Brunner G (2009) Near critical and supercritical water. Part I. Hydrolytic and hydrothermal processes. J Supercrit Fluids 47:373–381CrossRefGoogle Scholar
  3. Chen J, Liu G, Jin L, Ni P, Li Z, He H, Xu Y (2010) Catalytic hydrolysis of waste nylon 6 to produce ε-caprolactam in sub-critical water. J Anal Appl Pyrolysis 87:50–55CrossRefGoogle Scholar
  4. Daimon H, Kang K, Sato N, Fujie K (2001) Development of marine waste recycling technologies using sub- and supercritical water. J Chem Eng Jpn 34(9):1091–1096CrossRefGoogle Scholar
  5. Goto M, Obuchi R, Hirose T, Sakaki T, Shibata M (2004) Hydrothermal conversion of municipal organic waste into resources. Bioresour Technol 93:279–284PubMedCrossRefGoogle Scholar
  6. Jiang L, Hu F (2008) The research of conditions for acid hydrolyzation of soybean dregs. Food Res Dev 29(2):97–100 (Chinese)Google Scholar
  7. Kang K, Quitain AT, Daimon H, Noda R, Goto N, Hu H, Fujie K (2001a) Optimization of amino acids production from waste fish entrails by hydrolysis in sub- and supercritical water. Can J Chem Eng 79:65–70CrossRefGoogle Scholar
  8. Kang K, Quitain AT, Urano S, Daimon H, Fujie K (2001b) Rapid Sample injection in semi batch hydrothermal treatment of solid wastes. Ind Eng Chem Res 40:3717–3720CrossRefGoogle Scholar
  9. Katritzky AR, Nichols DA, Siskin M, Murugan R, Balasubramanian M (2001) Reactions in high-temperature aqueous media. Chem Rev 101:837–892PubMedCrossRefGoogle Scholar
  10. Kim K, Fujita M, Daimon H, Fujie K (2003) Application of hydrothermal reaction to biodegrade ability improvement of refractory pollutants: structural conversion of di- and trichloroacetic acid to biodegradable products. J Water Environ Technol 1(2):217–224CrossRefGoogle Scholar
  11. Klingler D, Berg J, Vogel H (2007) Hydrothermal reactions of alanine and glycine in sub- and supercritical water. J Supercrit Fluids 43:112–119CrossRefGoogle Scholar
  12. Li G, Kong L, Wang H, Huang J, Xu J (2008) Application of hydrothermal reaction in resource recovery of organic wastes. Resour Conserv Recycl 52:691–699CrossRefGoogle Scholar
  13. Li H, Yuan X, Zeng G, Tong J, Yan Y, Cao H, Wang L, Cheng M, Zhang J, Yang D (2009) Liquefaction of rice straw in sub- and supercritical 1,4-dioxane–water mixture. Fuel Process Technol 90:657–663CrossRefGoogle Scholar
  14. Miyoshi H, Chen D, Akai TA (2004) Novel process utilizing subcritical water to recycle soda-lime-silicate glass. J Non-Cryst Solids 337:280–282CrossRefGoogle Scholar
  15. Rogalinski T, Herrman S, Brunner G (2005) Production of amino acids from bovine serum albumin by continuous sub-critical water hydrolysis. J Supercrit Fluids 36(1):49–58CrossRefGoogle Scholar
  16. Sato N, Quitain AT, Kang K, Daimon H, Fujie K (2004) Reaction kinetics of amino acid decomposition in high-temperature and high-pressure water. Ind Eng Chem Res 43(13):3217–3222CrossRefGoogle Scholar
  17. Sereewatthanawut I, Prapintip S, Watchiraruji K, Goto M, Sasaki M, Shotipruk A (2008) Extraction of protein and amino acids from deoiled rice bran by subcritical water hydrolysis. Bioresour Technol 99:555–561PubMedCrossRefGoogle Scholar
  18. Shanableh A (2000) Production of useful organic matter from sludge using hydrothermal treatment. Water Res 34(3):945–951CrossRefGoogle Scholar
  19. Zhang L, Xu C, Champagne P (2010) Energy recovery from secondary pulp/paper-mill sludge and sewage with supercritical water treatment. Bioresour Technol 101(8):2713–2721PubMedCrossRefGoogle Scholar
  20. Zhu TJ, Zheng WW (2004) Soybean residues’ state of developing and prospects. Food Res Dev 25(4):25–27 (In Chinese)Google Scholar
  21. Zhu X, Zhu C, Zhao L, Cheng H (2008a) Amino acids production from fish proteins hydrolysis in subcritical water. Chin J Chem Eng 16(3):456–460CrossRefGoogle Scholar
  22. Zhu X, Cheng H, Zhu N (2008b) Reaction kinetics of fish meat hydrolysis for amino acids production in sub-critical water. Chem Eng (China) 36(4):31–33Google Scholar
  23. Zhu G-Y, Zhu X, Wan X-L, Fan Q, Ma Y-H, Qian J, Liu X-L, Shen Y-J, Jiang J-H (2010) Hydrolysis technology and kinetics of poultry waste to produce amino acids in subcritical water. J Anal Appl Pyrol 88:187–191CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  1. 1.Department of Chemical EngineeringShanghai UniversityShanghaiPeople’s Republic of China

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