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Amino Acids

, Volume 40, Issue 4, pp 1091–1106 | Cite as

Regulation of taurine homeostasis by protein kinase CK2 in mouse fibroblasts

  • Daniel Bloch Hansen
  • Barbara Guerra
  • Jack Hummeland Jacobsen
  • Ian Henry LambertEmail author
Original Article

Abstract

Increased expression of the ubiquitous serine/threonine protein kinase CK2 has been associated with increased proliferative capacity and increased resistance towards apoptosis. Taurine is the primary organic osmolyte involved in cell volume control in mammalian cells, and shift in cell volume is a critical step in cell proliferation, differentiation and induction of apoptosis. In the present study, we use mouse NIH3T3 fibroblasts and Ehrlich Lettré ascites tumour cells with different CK2 expression levels. Taurine uptake via the Na+ dependent transporter TauT and taurine release are increased and reduced, respectively, following pharmacological CK2 inhibition. The effect of CK2 inhibition on TauT involves modulation of transport kinetics, whereas the effect on the taurine release pathway involves reduction in the open-probability of the efflux pathway. Stimulation of PLA2 activity, exposure to exogenous reactive oxygen species as well as inhibition of protein tyrosine phosphotases (PTP) potentiate the swelling-induced taurine loss. Inhibition of PI3K and PTEN reduces and potentiates swelling-induced taurine release, respectively. Inhibition of CK2 has no effect on PLA2 activity and ROS production by NADPH oxidase, whereas it lifts the effect of PTEN and PTP inhibition. It is suggested that CK2 regulates the taurine release downstream to known swelling-induced signal transducers including PLA2, NADPH oxidase and PI3K.

Keywords

Regulatory volume decrease TBCA DMAT SLC6A6 Volume sensitive organic osmolyte channel 

Notes

Acknowledgments

The present work was supported by The Danish Council for Independent Research/Natural Sciences (Grant 272-07-0530, 272-08-0170, 272-07-0258), The Danish Council for Independent Research/Medical Sciences (Grant 271-08-0520), and The Danish Cancer Society (Grant DP08152). The technical assistance of Dorthe Nielsen is gratefully acknowledged.

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Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Daniel Bloch Hansen
    • 1
  • Barbara Guerra
    • 2
  • Jack Hummeland Jacobsen
    • 1
  • Ian Henry Lambert
    • 1
    Email author
  1. 1.Department of Biology, Section for Cell and Developmental BiologyUniversity of CopenhagenCopenhagen ØDenmark
  2. 2.Institute of Biochemistry and Molecular BiologyUniversity of Southern DenmarkOdense MDenmark

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