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Immunomodulatory activities of a new pentapeptide (Bursopentin) from the chicken bursa of Fabricius

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Abstract

The bursa of Fabricius (BF) is a central immune organ in birds, and some peptides from chicken BF have demonstrated important immune functions. Here, a new 626.27 Da pentapeptide, Bursopentin (BP5, Cys-Lys-Arg-Val-Tyr) was isolated and purified by reverse-phase high-performance liquid chromatography. In this study, we examined the effects of BP5 on antigen-specific immune response in BALB/c mice sensitized with inactivated avian influenza virus (AIV) [A/Duck/Jiangsu/NJ08/05 (AIV H9N2 subtype)]. The results suggested that BP5 enhanced anti-hemagglutinin antibody (IgG, the isotypes IgG1 and IgG2a) production, induced both of Th1- (IL-2 and IFN-γ) and Th2-type (IL-4 and -10) cytokines, increased proliferations of splenic lymphocyte subsets CD4+ T cells (CD3+CD4+), CD8+ T cells (CD3+CD8+) and B cells, and enhanced cytotoxic T-lymphocyte activity of the activated splenocytes against NIH3T3 cells. The effects of BP5 on the proliferation of isolated T- and/or B-cell populations of BALB/c mice were assessed, and the data suggested that BP5 promoted spleen lymphocyte proliferation by activating B cells directly and T cells indirectly. Further analysis revealed that B-lymphocyte proliferation induced by BP5 is mediated by reactive oxygen species generated from thiol auto-oxidation of BP5. Furthermore, our data indicated that protein kinase C, mitogen-activated protein kinase, and nuclear factor kappa B are involved in the signal transductions during the BP5-induced B lymphocyte proliferation. This study indicates that BP5 could be a potential immunomodulator for future immuno-pharmacological use.

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Acknowledgments

We thank Dr. Yingjuan Qian (University of California, Davis, USA) for his revision and discussion. We gratefully acknowledge Shanghai Gencore Biotechnologies Co., Ltd for providing Endman degradation analysis and China National Center of Biomedical Analysis for providing mass spectrometric analysis. This work was supported in part by grants from the National High-Tech Research and Development Program of the People’s Republic of China (863 Program) (2005AA246020).

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Authors and Affiliations

  1. Key Laboratory of Animal Disease Diagnosis and Immunology of Ministry of Agriculture of the People’s Republic of China, Nanjing Agricultural University, Nanjing, 210095, People’s Republic of China

    D. Y. Li & P. Y. Chen

  2. State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, People’s Republic of China

    Z. R. Geng

  3. Institute of Animal Husbandry and Veterinary Medicine, Chinese Academy of Agricultural Sciences, Beijing, People’s Republic of China

    H. F. Zhu

  4. College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, People’s Republic of China

    C. Wang

  5. Institute of Animal Husbandry and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, Shanghai, People’s Republic of China

    D. N. Miao

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  1. D. Y. Li
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  2. Z. R. Geng
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  3. H. F. Zhu
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  4. C. Wang
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  5. D. N. Miao
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Correspondence to P. Y. Chen.

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Li, D.Y., Geng, Z.R., Zhu, H.F. et al. Immunomodulatory activities of a new pentapeptide (Bursopentin) from the chicken bursa of Fabricius. Amino Acids 40, 505–515 (2011). https://doi.org/10.1007/s00726-010-0663-7

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  • DOI: https://doi.org/10.1007/s00726-010-0663-7

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