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Modulation of blackspot seabream (Pagellus bogaraveo) intermediary metabolic pathways by dispensable amino acids

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Abstract

The objective of the present work is to investigate the main metabolic pathways by which dispensable amino acids (DAA) are diverted towards lipid formation in blackspot seabream. For that purpose, a control diet was formulated to contain 45% of crude protein (7.2 g N/100 g dry matter) mainly supplied by fish meal (45P). In two other diets, 22.2% of the dietary nitrogen (1.6 g N/100 g dry matter) was replaced by an equivalent amount of nitrogen provided by two different mixtures of DAA: alanine and serine (diet AS) or aspartic and glutamic acid (diet AG). A fourth diet (diet 35P) only containing 35% of crude protein (5.6 g N/100 g dry matter) was included in order to analyze the possible additive effects of DAA. Compared to fish fed diet 35P, blackspot seabream appear to make a more efficient use of the nitrogen provided by alanine and serine than that provided by aspartic and glutamic acids in terms of growth. Contrary to fish fed AG, fish fed AS attained similar specific FAS activities as 45P fed fish, suggesting a further role of alanine and serine on this lipogenic pathway. Dietary nitrogen reduction (45P vs. 35P) or its replacement by a mixture of aspartic and glutamic acids (diet AG) were shown to up-regulate phosphoenolpyruvate carboxykinase (PEPCK) but without, however, any effect on plasma glucose levels. Dietary nitrogen level and nature seems to exert a complex regulation on energetic pathways through the gluconeogenesis/tricarboxylic acids cycle interaction.

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Abbreviations

AA:

Amino acids

CHOL:

Cholesterol

DAA:

Dispensable amino acids

IAA:

Indispensable amino acids

FAA:

Free amino acids

FA:

Fatty acid

FAs:

Fatty acids

HSI:

Hepatosomatic index

MUFA:

Monounsaturated fatty acids

PUFA:

Polyunsaturated fatty acids

TAG:

Triacylglycerol

VSI:

Viscerosomatic index

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Acknowledgments

This work was supported by “Optidietas project” (Agência de Inovação, Portugal, with the support of the European fund FEDER). A. Cláudia Figueiredo-Silva was supported by FCT, Fundação para a Ciência e Tecnologia of Portugal (PhD Grant SFRH/BD/22401/2005). Special thanks to L. Larroquet, C. Vachot, M.J. Borthaire, J. Breque, and António Júlio Pinto for technical assistance.

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  1. CIMAR/CIIMAR-Centro Interdisciplinar de Investigação Marinha e Ambiental, and ICBAS-Instituto de Ciências Biomédicas de Abel Salazar, Universidade do Porto, Rua dos Bragas, 289, 4050-123, Porto, Portugal

    A. Cláudia Figueiredo-Silva & Luísa M. P. Valente

  2. INRA-UMR Nutrition Aquaculture Génomique, Pôle Hydrobiologie, 64310, Saint Pée-sur-Nivelle, France

    A. Cláudia Figueiredo-Silva, Geneviève Corraze & Sadasivam Kaushik

  3. IEO-Instituto Español de Oceanografia, Centro Oceanográfico de Vigo, Cabo Estay-Canido, 1552, 36280, Vigo, Spain

    José B. Peleteiro

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  1. A. Cláudia Figueiredo-Silva
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  2. Geneviève Corraze
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  3. Sadasivam Kaushik
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Correspondence to Luísa M. P. Valente.

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Figueiredo-Silva, A.C., Corraze, G., Kaushik, S. et al. Modulation of blackspot seabream (Pagellus bogaraveo) intermediary metabolic pathways by dispensable amino acids. Amino Acids 39, 1401–1416 (2010). https://doi.org/10.1007/s00726-010-0599-y

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