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Peptidomic analysis of the skin secretions of the frog Pachymedusa dacnicolor

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Abstract

High-resolution mass spectrometry-based peptidomics has been used to characterize several components in electro-stimulated skin secretions of the endemic Mexican frog Pachymedusa dacnicolor. Peptide mass screening performed in an Orbitrap-XL mass spectrometer showed that P. dacnicolor skin secretions possess 194 different components with molecular masses ranging mainly from 500 to 6,000 Da. Dozens of molecules were partially sequenced including two novel protease inhibitors. Additionally, one posttranslationally modified bradykinin and two novel dermaseptin-like antimicrobial peptides were fully sequenced. The novel peptide named here DMS-DA5 was fully characterized and showed potent antibacterial activity against various bacteria such as Escherichia coli, Bacillus subtilis, Salmonella enterica serovar typhimurium, and Pseudomonas aeruginosa with minimal inhibitory concentrations from 3.10 to 25.0 μM.

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Acknowledgments

This research was supported by grant from the Dirección General de Asuntos del Personal Académico (DGAPA) of the National Autonomous University of Mexico-UNAM (Grant number IN221508 to CVFB).

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Authors and Affiliations

  1. Unidad de Proteómica-Instituto de Biotecnología, Universidad Nacional Autónoma de México (UNAM), Av. Universidad, 2001 Col. Chamilpa, CP 62210, Cuernavaca, Morelos, Mexico

    Erika P. Meneses, Oscar Villa-Hernández, Lorena Hernández-Orihuela & Cesar Vicente Ferreira Batista

  2. Lab. Herpetología, Centro de Investigaciones Biológicas, UAEM, Av. Universidad 1001, Col. Chamilpa, CP 62209, Cuernavaca, Morelos, Mexico

    Ruben Castro-Franco

  3. Centro de Investigación Sobre Enfermedades Infecciosas, Instituto Nacional de Salud Pública, Av. Universidad No. 655. Col. Santa María Ahuacatitlán, Cerrada Los Pinos y Caminera, CP 62100, Cuernavaca, Morelos, Mexico

    Victoria Pando

  4. Laboratorio de Neurofarmacología Marina, Departamento de Neurobiologia Celular y Molecular, Instituto de Neurobiología, UNAM, Campus Juriquilla, Boulevar Juriquilla 3001, 76230, Juriquilla, Qro., Mexico

    Manuel B. Aguilar

Authors
  1. Erika P. Meneses
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  2. Oscar Villa-Hernández
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  3. Lorena Hernández-Orihuela
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  4. Ruben Castro-Franco
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  5. Victoria Pando
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  6. Manuel B. Aguilar
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  7. Cesar Vicente Ferreira Batista
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Correspondence to Cesar Vicente Ferreira Batista.

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Meneses, E.P., Villa-Hernández, O., Hernández-Orihuela, L. et al. Peptidomic analysis of the skin secretions of the frog Pachymedusa dacnicolor . Amino Acids 40, 113–122 (2011). https://doi.org/10.1007/s00726-010-0564-9

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  • DOI: https://doi.org/10.1007/s00726-010-0564-9

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