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Effects of zinc ex vivo and intracellular zinc chelator in vivo on taurine uptake in goldfish retina

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Abstract

Taurine and zinc exert neurotrophic effects. Zinc modulates Na+/Cl−-dependent transporters. This study examined the effect of zinc (ZnSO4) ex vivo and zinc chelator N,N,N′,N′-tetrakis-(2-pyridylmethyl) ethylenediamine (TPEN) in vivo on [3H]taurine transport in goldfish retina. The effect of TPEN in vivo on taurine and zinc levels was determined. Isolated cells were incubated in Ringer with zinc (0.1–100 μM). Taurine transport was done with taurine (0.001–1 mM) and 50 nM [3H]taurine. Zinc (100 μM) noncompetitively inhibited taurine transport. TPEN was administered intraocularly and retinas extracted 3, 5 and 10 days later. Taurine was determined by HPLC (nmol/mg protein) and zinc by spectrophotometry ICP (mg/mg protein). Taurine and zinc levels decreased at 3 days and increased at 10 days after TPEN administration. At 10 days after intraocular TPEN, taurine transport affinity increased (K s = 0.018 ± 0.006 vs. 0.028 ± 0.008 mM). Apparently, zinc deficiency affects the taurine–zinc complex and taurine availability. The increased taurine uptake affinity by TPEN was possibly associated with a response to maximize retinal taurine content at low zinc concentration.

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Abbreviations

DMSO:

Dimethyl sulfoxide

i.o.:

Intraocular injection

EC50 :

Mean effective concentration

TPEN:

N,N,N′,N′-tetrakis-(2-pyridylmethyl) ethylenediamine

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Acknowledgments

This work was supported by Grant S1-2001-903 from Fondo Nacional de Investigación, Tecnología e Innovación (FONACIT), Venezuela. S. Nusetti was a Ph.D. student of Centro de Estudios Avanzados, Instituto Venezolano de Investigaciones Científicas, with financial support from Universidad de Oriente and FONACIT.

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  1. S. Nusetti

    Present address: Postgrado de Biología Aplicada, Cerro del Medio, Núcleo de Sucre, Universidad de Oriente, Cumaná, Venezuela

Authors and Affiliations

  1. Laboratorio de Neuroquímica, Centro Biofísica Bioquímica, Instituto Venezolano de Investigaciones Científicas, Caracas, Venezuela

    S. Nusetti, M. Urbina, F. Obregón, M. Quintal, Z. Benzo & L. Lima

  2. Laboratorio de Química Analítica, Centro de Química, Instituto Venezolano de Investigaciones Científicas, Caracas, Venezuela

    M. Quintal & Z. Benzo

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  1. S. Nusetti
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Correspondence to L. Lima.

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Nusetti, S., Urbina, M., Obregón, F. et al. Effects of zinc ex vivo and intracellular zinc chelator in vivo on taurine uptake in goldfish retina. Amino Acids 38, 1429–1437 (2010). https://doi.org/10.1007/s00726-009-0357-1

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