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Effects of 2-hydroxy-4-methylthiobutyrate on portal plasma flow and net portal appearance of amino acids in piglets

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Abstract

To determine whether portal plasma flow (PPF) and net portal appearance of amino acids (AA) could be affected by 2-hydroxy-4-methylthiobutyrate (HMB), six barrows (35-day-old, 8.6 ± 1.4 kg), implanted with arterial, portal and mesenteric catheters, were fed a dl-methionine (as the control) or HMB-supplemented diet once hourly and infused intramesenterically with 1% p-amino hippurate. PPF was numerically 9% higher (P = 0.09) in HMB-fed pigs than in controls over a 4–6 h period. Compared with controls, pigs fed the HMB diet had increased (P < 0.05) net portal balance and/or appearance of leucine, isoleucine, histidine, arginine and alanine, but had decreased (P < 0.05) portal appearance of glutamate over a 6-h period. The concentration of acetate in the lumen of the distal small intestine was higher (P = 0.01) in HMB-fed pigs than in controls (25.14 vs. 7.64 mmol/kg). mRNA levels for proglucagon and endothelial nitric-oxide synthase (eNOS) in stomach and proximal small intestine, and mRNA levels for GLP-2 receptor (GLP-2R) in stomach were higher (P < 0.05) in HMB-fed pigs compared with those in controls. Collectively, HMB supplementation increased concentrations of short-chain fatty acids in intestinal lumen, expression of proglucagon, GLP-2R, and eNOS genes, and net portal absorption of AA. These novel findings from the study with pigs may also have important implications for intestinal nutrition and health in humans.

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Acknowledgments

We express our gratitude to Prof. Guoyao Wu at Texas A&M University for his kind assistance in the editing of this article. We also thank Kang Yao, Zhihong Sun, and Zhenli Liu for technical assistance. This work was supported by the National Natural Science Foundation of China (No. 30771560) and the Natural Science Foundation of Hubei Province (No. 4006-066067).

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Authors and Affiliations

  1. Department of Animal Nutrition, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    Z. F. Fang, J. Luo, Z. L. Qi, F. R. Huang, M. Y. Liu & J. Peng

  2. Key Laboratory of Swine Breeding and Genetics of Agricultural Ministry, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    S. W. Jiang

  3. Department of Feed Science, Wuhan Polytechnic University, Wuhan, 430023, People’s Republic of China

    S. J. Zhao

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  1. Z. F. Fang
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  2. J. Luo
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Correspondence to S. W. Jiang or J. Peng.

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Fang, Z.F., Luo, J., Qi, Z.L. et al. Effects of 2-hydroxy-4-methylthiobutyrate on portal plasma flow and net portal appearance of amino acids in piglets. Amino Acids 36, 501–509 (2009). https://doi.org/10.1007/s00726-008-0110-1

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  • DOI: https://doi.org/10.1007/s00726-008-0110-1

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