Skip to main content
SpringerLink
Log in

Kinetic determination of tryptophan by using the B-Z oscillating chemical system

  • Original Article
  • Published:
Amino Acids Aims and scope Submit manuscript

Abstract

A simple and rapid method was devised for determination of tryptophan, based on the Belousov-Zhabotinskii (B-Z) oscillating chemical system. Changes in oscillating period and amplitude were linearly proportional to the negative logarithm of l-tryptophan concentration over the range of 6.44 × 10−7–2.55 × 10−4 M, with the regression coefficients of near unity and a lower detection limit of 6.5 × 10−8 M. d-tryptophan was also examined although it is rarely found in most biological fluids, and perhaps not at all in natural proteins. The change of period against to negative logarithm of d-tryptophan concentration over the range of 4.9 × 10−5–8.24 × 10−4 M is linear. Because the optimum conditions for determination of l- and d-tryptophan are not the same, a little amount of d-tryptophan does not affect the determination of l-tryptophan. Various influences were studied and a possible mechanism of perturbation to the B-Z oscillator by tryptophan was also discussed. Spectrophotometry and fluorescence spectrophotofluorimetry were used for comparision and confirmation of the results.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Alwarthan AA (1995) Chemiluminescent determination of tryptophan in a flow injection system. Anal Chim Acta 317:233–237

    Article  CAS  Google Scholar 

  • Field RJ, Schneider FW (1989) Oscillating chemical reactions and nonlinear dynamics. J Chem Educ 66:195–204

    Article  CAS  Google Scholar 

  • Gao JZ (2005) Application of oscillating chemical reaction to analytical chemistry: recent developments. Pak J Biol Sci 8:512–519

    Article  CAS  Google Scholar 

  • Gao JZ, Ren J, Yang W, Liu XH, Yang H, Li QZ, Deng HL (2002) Kinetic determination of hydroquinone by Belousov-Zhabotinsky oscillating chemical reaction. J Electroanal Chem 520:157–161

    Article  CAS  Google Scholar 

  • Gao JZ, Chen H, Dai HX, Lv DY, Ren J, Wang L, Yang W (2006a) Improved sensitivity for transition metal ions by use of sulfide in the Belousov-Zhabotinskii oscillating reaction. Anal Chim Acta 571:150–155

    Article  PubMed  CAS  Google Scholar 

  • Gao JZ, Dai HX, Yang W, Chen H, Lv DY, Ren J, Wang L (2006b) Determination of furfural by oscillating chemical reaction using an analyte pulse perturbation technique. Anal Bioanal Chem 384:1438–1443

    Article  PubMed  CAS  Google Scholar 

  • Gao JZ, Wang L, Yang W, Yang FW (2006c) Kinetic determination of indium ion based on the B-Z oscillating chemical system. J Braz Chem Soc 17:458–462

    CAS  Google Scholar 

  • Guo YJ (1987) Spectrophotometry and its application in biochemistry (Ch). Science Press, Beijing, pp 223–231

    Google Scholar 

  • Hanaoka S, Lin JM, Yamada M (2000) Chemiluminescence behavior of the decomposition of hydrogen peroxide catalyzed by copper(II)–amino acid complexes and its application to the determination of tryptophan and phenylalanine. Anal Chim Acta 409:65–73

    Article  CAS  Google Scholar 

  • Jimenez-Prieto R, Silva M, Perez-Bendito D (1998) Approaching the use of oscillating reactions for analytical monitoring. Analyst 123:1R–8R

    Article  CAS  Google Scholar 

  • Masahiro S, Michael HD, Michael TP (2001) Chiral polyoxotungstates. 1. Stereoselective interaction of amino acids with enantiomers of [CeIII(r1-P2W17O61)(H2O)x]7-. The structure of DL-[Ce2(H2O)8(P2W17O61)2]14−. Inorg Chem 40:2715–2719

    Article  CAS  Google Scholar 

  • Raoof JB, Ojani R, Kiani A, Khosravi M, Adnani A (2005) The potentiometric effect of hydrazine on a B-Z oscillating chemical reaction: application to the determination of hydrazine. Bull Chem Soc Jpn 78:258–261

    Article  CAS  Google Scholar 

  • Strizhak PE, Khavrus VO (2000) Determination of gases (NO, CO, Cl2) using mixed-mode regimes in the Belousov-Zhabotinskii oscillating chemical reaction. Talanta 51:935–947

    Article  PubMed  CAS  Google Scholar 

  • Strizhak PE, Didenko OZ, Ivashchenko TS (2001) Determination of traces of thallium using the transient chaotic regime in the Belousov-Zhabotinskii oscillating chemical reaction. Anal Chim Acta 428:15–21

    Article  CAS  Google Scholar 

  • Taylor AF (2002) Mechanism and phenomenology of an oscillating chemical reaction. Progr React Kinet Mech 27:247–325

    CAS  Google Scholar 

  • Toledo R, Silva M, Khavrus VO, Strizhak PE (2000) Potential of the analyte pulse perturbation technique for the determination of polyphenols based on the Belousov-Zhabotinskii reaction. Analyst 125:2118–2124

    Article  CAS  Google Scholar 

  • Wu F, Guo WD, Zhang R, Xia EQ, Zhou KB (2005) Fluorescence measurement of tryptophan content in microalgae. Marine Sci (Ch) 29:1–4

    CAS  Google Scholar 

  • Xu SC (1982) Orgc chem (Ch). People’s Educational Press, Beijing, pp 385–401

    Google Scholar 

  • Yang X, Mei XG, Wang AS (2002) Direct determination of free aromatic amino acid in suspension of taxus chinensis cell by high performance capillary eletrophoresis. Amino Acid Bio Res (Ch) 24:50–53

    Google Scholar 

  • Yust MM, Pedroche J, Giron-Calle J (2004) Determination of tryptophan by high performance liquid chromatography of alkaline hydrolysates with spectrophotometric detection. Food Chem 85:317–320

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported in part by the Project of International Cooperation between China and Ukraine (043−05), the National Natural Science Foundation (20475044) and the project of KJCXGC-01, NWNU, China.

Author information

Authors and Affiliations

  1. College of Chemistry and Chemical Engineering, Northwest Normal University, 730070, Lanzhou, People’s Republic of China

    Jinzhang Gao, Jie Qu, Wu Yang, Xiaoxia Wei, Hongxia Dai, Dongyu Lv, Jie Ren & Hua Chen

Authors
  1. Jinzhang Gao
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jie Qu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Wu Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Xiaoxia Wei
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Hongxia Dai
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Dongyu Lv
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Jie Ren
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Hua Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jinzhang Gao.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gao, J., Qu, J., Yang, W. et al. Kinetic determination of tryptophan by using the B-Z oscillating chemical system. Amino Acids 36, 391–397 (2009). https://doi.org/10.1007/s00726-008-0064-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00726-008-0064-3

Keywords

Search

Navigation