Skip to main content
SpringerLink
Log in

Proteome analysis of soybean hypocotyl and root under salt stress

  • Original Article
  • Published:
Amino Acids Aims and scope Submit manuscript

Abstract

To evaluate the response of soybean to salt stress, the related changes in protein expression were investigated using the proteomic approach. Soybean plants were exposed to 0, 50, 100, and 200 mM NaCl. Especially at 200 mM, the length and fresh weight of the hypocotyl and root reduced under salt stress, while the proline content increased. Proteins from the hypocotyl and root treated with 100 mM NaCl were extracted and separated by two-dimensional polyacrylamide gel electrophoresis; 321 protein spots were detected. In response to salt stress, seven proteins were reproducibly found to be up- or down-regulated by two to sevenfold: late embryogenesis-abundant protein, β-conglycinin, elicitor peptide three precursor, and basic/helix-loop-helix protein were up-regulated, while protease inhibitor, lectin, and stem 31-kDa glycoprotein precursor were down-regulated. These results indicate that salinity can change the expression level of some special proteins in the hypocotyl and root of soybean that may in turn play a role in the adaptation to saline conditions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

Abbreviations

2D-PAGE:

Two-dimensional polyacrylamide gel electrophoresis

References

  • Abbasi FM, Komatsu S (2004) A proteomic approach to analyze salt-responsive proteins in rice leaf sheath. Proteomics 4:2072–2081

    Article  PubMed  CAS  Google Scholar 

  • Amini F, Ehsanpour A A, Hoang QT Shin JS (2007) Protein pattern changes in tomato under in vitro salt stress. Russ J Plant Physiol 54:464–471

    Article  CAS  Google Scholar 

  • Amitai-Zeigersona H, Scolnikb PA, Bar-Zvi D (1995) Tomato Asrl mRNA and protein are transiently expressed following salt stress, osmotic stress and treatment with abscisic acid. Plant Sci 110:205–213

    Article  Google Scholar 

  • Baker J, Steele C, Dure L (1988) Sequence and characterization of 6 Lea proteins and their genes from cotton. Plant Mol Biol 11:277–291

    Article  CAS  Google Scholar 

  • Banzai T, Hershkovits G, Katcoff DJ, Hanagata N, Dubinsky Z, Karube I (2002) Identification and characterization of mRNA transcrips differentially expressed in response to high salinity by means of differential display in the mangrove, Bruguiera gymnorrhiza. Plant Sci 162:499–505

    Article  CAS  Google Scholar 

  • Bates L, Waldren RP, Tear IP (1973) Rapid determination of free proline for water stress studies. Plant Soil 39:205–207

    Article  CAS  Google Scholar 

  • Benevides MP, Marconi LP, Gallego SM, Comba ME, Tomaro ML (2000) Relationship between antioxidant defense system and salt tolerance in Solanum tuberosum. Aus J Plant Physiol 27:273–278

    Google Scholar 

  • Castro AJ, Carapito C, Zorn N, Magne C, Leize E, Dorsselaer AV (2005) Proteomic analysis of grapevine (Vitis vinifera L.) tissues subjected to herbicide stress. J Exp Bot 56:2783–2795

    Article  PubMed  CAS  Google Scholar 

  • Chandler PM, Robertson M (1994) Gene expression regulated by abscisic acid and its relation to stress tolerance. Annu Rev Plant Physiol Plant Mol Biol 45:113–141

    Article  CAS  Google Scholar 

  • Cleveland DW, Fischer SG, Krischer MW, Laemmli UK (1977) Peptide mapping by limited proteolysis in sodium dodecyl sulphate and analysis by gel electrophoresis. J Biol Chem 252:1102–1106

    PubMed  CAS  Google Scholar 

  • Ehsanpour AA, Fatahian N (2003) Effect of salt and proline on Medicago sativa callus. Plant Cell Tissue Organ Cult 73:53–56

    Article  CAS  Google Scholar 

  • Habib H, Fazili KM (2007) Plant protease inhibitors: a defense strategy in plants. Biotech Mol Biol Rev 2:68–85

    Google Scholar 

  • Hajduch M, Rakwal R, Agrawal GK, Yonekura M, Pretova A (2001) High resolution two-dimensional electrophoresis separation of proteins from metal-stressed rice (Oryza sativa L.) leaves: drastic reductions/fragmentation of ribulose-1, 5-bisphosphate carboxylase/oxygenase and induction of stress-related proteins. Electrophoresis 22:2824–2831

    Article  PubMed  CAS  Google Scholar 

  • Honee G, Buitink J, Jabs T, De Kloe J, Sijbolts F, Apotheker M, Weide R, Sijen T, Stuiver M, De Wit PJGM (1998) Induction of defense-related responses in Cf9 tomato cells by the AVR9 elicitor peptide of Cladosporium fulvum is developmentally regulated. Plant Physiol 117:809–820

    Article  PubMed  CAS  Google Scholar 

  • Jelloulia N, Jouiraa HB, Skourib H, Ghorbela A, Gourgourib A, Mlikia A (2007) Proteomic analysis of Tunisian grapevine cultivar Razegui under salt stress. J Plant Physiol (on line)

  • Kao WY, Tsai TT, Tsai HC, Shih CN (2006) Response of three Glycine species to salt stress. Environ Exp Bot 56:120–125

    Article  CAS  Google Scholar 

  • Katerji N, Van Hoornb JW, Hamdyc A, Mastrorillid M (2000) Salt tolerance classification of crops according to soil salinity and to water stress day index. Agric Water Manag 43:99–109

    Article  Google Scholar 

  • Kong-Ngern K, Daduang S, Wongkham CH, Bunnag S, Kosittrakun M, Theerakulpisut P (2005) Protein profiles in response to salt stress in leaf sheaths of rice seedlings. Sci Asia 31:403–408

    Article  CAS  Google Scholar 

  • Krishman HB (2002) Evidence for accumulation of the β-subunit of β-conglycinin in soybean [Glycine max (L) Merr.] embryonic axes. Plant Cell Rep 20:869–875

    Article  CAS  Google Scholar 

  • Li D, Inoue H, Takahashi M, Kojima T, Shiraiwa M, Takahara H (2007) Molecular characterization of a novel salt inducible gene for an OSBP (oxysterol-binding protein)-homologue from soybean. Gene (on line)

  • Lu Z, Liu D, Liu S (2007) Two rice cytosolic ascorbate peroxidases differentially improve salt tolerance in transgenic Arabidopsis. Plant Cell Rep (on line)

  • Luo Q, Yu B, Liu Y (2005) Differential sensitivity to chloride and sodium ions in seedlings of Glycine max and G. soja under NaCl stress. J Plant Physiol 162:1003–1012

    PubMed  CAS  Google Scholar 

  • Martienz CA, Maestri M, Lani EG (1996) In vitro salt tolerance and proline accumulation in Andean potato (Solanum spp.) differing in frost resistance. Plant Sci 116:177–184

    Article  Google Scholar 

  • Mittova V, Guy M, Tal M, Volokita M (2004) Salinity up-regulates the antioxidative system in root mithochondria and peroxisomes of wild salt-tolerant tomato species Lycopersicon pennellii. J Exp Bot 55:1105–1113

    Article  PubMed  CAS  Google Scholar 

  • NDong C, Danyluk J, Wilson KE, Pocock T, Huner NP, Sarhan F (2002) Cold-regulated cereal chloroplast late embryogenesis abundant-like proteins: molecular characterization and functional analyses. Plant Physiol 129:1368–1381

    Article  PubMed  CAS  Google Scholar 

  • O’Farrell HP (1975) High resolution two-dimensional electrophoresis of proteins. J Biol Chem 250:4007–4021

    PubMed  CAS  Google Scholar 

  • Onishi M, Tachi H, Kojima T, Shiraiwa M, Takahara H (2006) Molecular cloning and characterization of a novel salt inducible gene encoding an acidic isoform of PR-5 protein in soybean (Glycine max [L.] Merr). Plant Physiol Biochem 44:573–580

    Article  CAS  Google Scholar 

  • Parker R, Flowers TJ, Moore AL, Harpham NVJ (2006) An accurate and reproducible method for proteome profiling of the effects of salt stress in the rice leaf lamina. J Exp Bot 57:1109–1118

    Article  PubMed  CAS  Google Scholar 

  • Peumans Willy J, Van Damme EJM (1995) Lectins as plant defense proteins. Plant Physiol 109:347–352

    Article  Google Scholar 

  • Rakwal R, Agrawal GK, Yonekura M (1999) Separation of proteins from stressed rice (Oryza sativa L.) leaf tissues by two-dimensional polyacrylamide gel electrophoresis: induction of pathogenesis-related and cellular protect proteins by jasmonic acid, UV irradiation and copper chloride. Electrophoresis 20:3472–3478

    Article  PubMed  CAS  Google Scholar 

  • Sharifia M, Ghorbanlib M, Ebrahimzadeh H (2007) Improved growth of salinity-stressed soybean after inoculation with salt pre-treated mycorrhizal fungi. J Plant Physiol 164:1144–1151

    Article  CAS  Google Scholar 

  • Sharp RE, Hsiao TC, Silk WK (1990) Growth of maize primary root at low water potentials. Plant Physiol 93:1337–1346

    Article  PubMed  CAS  Google Scholar 

  • Sha Valli Khan PS, Hoffmann L, Renaut J, Hausman JF (2007) Current initiatives in proteomics for the analysis of plant salt tolerance. Curr Sci 93:807–817

    Google Scholar 

  • Soulages JL, Kim K, Walters C, Cushman JC (2002) Temperature-induced extended helix/random coil transitions in a group 1 late embryogenesis-abundant protein from soybean. Plant Physiol 128:822–832

    Article  PubMed  CAS  Google Scholar 

  • Srivastava S, Fristensky B, Kav NNV (2004) Constitutive expression of a PR10 protein enhances the germination of Brassica napus under saline conditions. Plant Cell Physiol 45:1320–1324

    Article  PubMed  CAS  Google Scholar 

  • Sule A, Vanrobaeys F, Hajos J, Beeumen JV, Devreese B (2004) Proteomic analysis of small heat shock protein isoforms in barley shoots. Phytochem 65:1853–1863

    Article  CAS  Google Scholar 

  • Toledo-Orits G, Huq E, Quail PH (2003) The Arabidopsis basic/helix-loop-helix transcription factor family. Plant Cell 15:1749–1770

    Article  CAS  Google Scholar 

  • Umezawa T, Shimuzu K, Kato M, Ueda T (2000) Enhancement of salt tolerance in soybean with NaCl pretreatment. Physiol Plant 110:59–63

    Article  CAS  Google Scholar 

  • Van Hardeen PDR Kruger GHJ (2002) Separately and simultaneously induced dark chilling and drought stress effects on photosynthesis, proline accumulation and antioxidant metabolism in soybean. J Plant Physiol 159:1077–1086

    Article  Google Scholar 

  • Vincent D, Lapierre C, Pollet B, Cornic G, Negroni L, Zivy M (2005) Water deficits affect caffeate O-methyltransferase, lignification, and related enzymes in maize leaves: a proteomic investigation. Plant Physiol 3:949–960

    Article  CAS  Google Scholar 

  • Wang M, Gu D, Liu T, Wang Z, Guo Xiying, Hou W, Bai Y, Chen X, Wang G (2007) Overexpression of a putative maize calcineurin B-like protein in Arabidopsis confers salt tolerance. Plant Mol Biol (on line)

  • Xu D, Duan X, Wang B, Hong B, Ho THD, Wu R (1996) Expression of a late embryogenesis abundant protein gene, HVA7, from barley confers tolerance to water deficit and salt stress in transgenic rice. Plant Physiol 110:249–257

    PubMed  CAS  Google Scholar 

Download references

Acknowledgments

The authors are grateful to scholarship section of the ministry of Science, Research and technology of I. R. Iran and the higher education department of Isfahan University and also thank to the National Institute of Crop Science of Japan for their kindly supports.

Author information

Authors and Affiliations

  1. National Institute of Crop Science, Kannondai 2-1-18, Tsukuba, 305-8518, Japan

    K. Aghaei, A. H. Shah & S. Komatsu

  2. Department of Biology, Faculty of Sciences, Isfahan University, Isfahan, Iran

    K. Aghaei & A. A. Ehsanpour

Authors
  1. K. Aghaei
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. A. Ehsanpour
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. H. Shah
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. S. Komatsu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to S. Komatsu.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Aghaei, K., Ehsanpour, A.A., Shah, A.H. et al. Proteome analysis of soybean hypocotyl and root under salt stress. Amino Acids 36, 91–98 (2009). https://doi.org/10.1007/s00726-008-0036-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00726-008-0036-7

Keywords

Search

Navigation