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Amino Acids

, Volume 35, Issue 2, pp 359–364 | Cite as

The role of heme oxygenase-1 in down regulation of PGE2 production by taurine chloramine and taurine bromamine in J774.2 macrophages

  • R. Olszanecki
  • M. Kurnyta
  • R. Biedroń
  • P. Chorobik
  • M. Bereta
  • J. Marcinkiewicz
Article

Summary.

Taurine chloramine (TauCl) and taurine bromamine (TauBr), products of myeloperoxidase halide system, exert anti-inflammatory properties. TauCl was demonstrated to inhibit the production of a variety of pro-inflammatory mediators including cyclooxygenase-2 (COX-2) dependent production of prostaglandin E2 (PGE2). Recently we have demonstrated that both major leukocyte haloamines, TauCl and TauBr, induced expression of HO-1 in non-activated and LPS-activated J774.2 macrophages. In this study, we have shown that TauCl and TauBr, at non-cytotoxic concentrations, inhibited the production of (PGE2) without altering the expression of COX-2 protein, in LPS/IFN-γ stimulated J774.2 cells. The inhibitory effect of TauCl and TauBr was reversed by chromium III mesoporhyrin (CrMP), an inhibitor of HO-1 activity. Our data suggest that HO-1 might participate in anti-inflammatory effects of TauCl/TauBr possibly by inhibition of COX-2 activity and decrease of PGE2 production.

Keywords: Inflammation – Macrophages – Heme oxygenase-1 – Taurine bromamine – Taurine chloramine – Prostaglandin E2 – Cyclooxygenase-2 

Abbreviations:

COX-2

Cyclooxygenase-2

DMSO

dimethyl sulfoxide

MTT

3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide

FCS

fetal calf serum

HO-1

heme oxygenase-1

IFNγ

interferon γ

IL-6

interleukin-6

IL-8

interleukin-8

IL-10

interleukin-10

IL-12

interleukin-12

iNOS

inducible nitric oxide synthase

LPS

lipopolysaccharide

PBS

phosphate buffered saline

PD 98059

2′-amino-3′-methoxyflavone

PGE2

prostaglandin E2

PGJ2

prostaglandin J2

ROS

reactive oxygen species

RT

room temperature

SB 203580

4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)1H-imidazole-

SDS

dodecyl sodium sulfate

TauBr

taurine bromamine

TauCl

taurine chloramine

TGF-β

tumor growth factor-β

TNF-α

tumor necrosis factor

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • R. Olszanecki
    • 2
  • M. Kurnyta
    • 1
  • R. Biedroń
    • 1
  • P. Chorobik
    • 1
  • M. Bereta
    • 1
  • J. Marcinkiewicz
    • 1
  1. 1.Chair of Immunology, Jagiellonian University Medical CollegeKrakówPoland
  2. 2.Chair of Pharmacology, Jagiellonian University Medical CollegeKrakówPoland

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