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Amino Acids

, Volume 35, Issue 2, pp 267–274 | Cite as

Functional proteomics to identify critical proteins in signal transduction pathways

  • G.-R. Yan
  • Q.-Y. He
Review Article

Summary.

Reversible protein phosphorylation plays a crucial role in the regulation of signaling pathways that control various biological responses, such as cell growth, differentiation, invasion, metastasis and apoptosis. Proteomics is a powerful research approach for fully monitoring global molecular responses to the activation of signal transduction pathways. Identification of different phosphoproteins and their phosphorylation sites by functional proteomics provides informational insights into signaling pathways triggered by all kinds of factors. This review summarizes how functional proteomics can be used to answer specific questions related to signal transduction systems of interest. By examining our own example on identifying the novel phosphoproteins in signaling pathways activated by EB virus-encoded latent membrane protein 1 (LMP1), we demonstrated a functional proteomic strategy to elucidate the molecular activity of phosphorylated annexin A2 in LMP1 signaling pathway. Functional profiling of signaling pathways is promising for the identification of novel targets for drug discovery and for the understanding of disease pathogenesis.

Keywords: Proteomics – Phosphoproteomics – Signal transduction – Phosphorylation 

Abbreviations:

IMAC

immobilized metal affinity chromatography

LMP1

latent membrane protein 1

PDGF

platelet-derived growth factor

PKC

protein kinase C

PMAC

phosphate metal affinity chromatography

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • G.-R. Yan
    • 1
  • Q.-Y. He
    • 1
  1. 1.Institutes of Life and Health EngineeringJinan UniversityGuangzhouChina

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