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Amino acids supply in culture media is not a limiting factor in the matrix synthesis of engineered cartilage tissue

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Summary.

Increased amino acid supplementation (0.5×, 1.0×, and 5.0× recommended concentrations or additional proline) was hypothesized to increase the collagen content in engineered cartilage. No significant differences were found between groups in matrix content or dynamic modulus. Control constructs possessed the highest compressive Young’s modulus on day 42. On day 42, compared to controls, decreased type II collagen was found with 0.5×, 1.0×, and 5.0× supplementation and significantly increased DNA content found in 1.0× and 5.0×. No effects were observed on these measures with added proline. These results lead us to reject our hypothesis and indicate that the low collagen synthesis in engineered cartilage is not due to a limited supply of amino acids in media but may require a further stimulatory signal. The results of this study also highlight the impact that culture environment can play on the development of engineered cartilage.

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Authors and Affiliations

  1. Cellular Engineering Laboratory, Department of Biomedical Engineering, Columbia University, New York, NY, USA

    K. W. Ng, L. E. Kugler, T.-A. N. Kelly, M. M. Ho, C. J. O’Conor & C. T. Hung

  2. Department of Orthopedic Surgery, St. Luke’s – Roosevelt Hospital Center, New York, NY, USA

    J. G. DeFrancis

  3. Musculoskeletal Biomechanics Laboratory, Departments of Biomedical and Mechanical Engineering, Columbia University, New York, NY, USA

    G. A. Ateshian

Authors
  1. K. W. Ng
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  2. J. G. DeFrancis
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  3. L. E. Kugler
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  4. T.-A. N. Kelly
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  5. M. M. Ho
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  6. C. J. O’Conor
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  7. G. A. Ateshian
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  8. C. T. Hung
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Authors’ address: Clark T. Hung, Ph.D., Cellular Engineering Laboratory, Department of Biomedical Engineering, Columbia University, 351 Engineering Terrace, MC8904, 1210 Amsterdam Avenue, New York, NY 10027, USA

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Ng, K., DeFrancis, J., Kugler, L. et al. Amino acids supply in culture media is not a limiting factor in the matrix synthesis of engineered cartilage tissue. Amino Acids 35, 433–438 (2008). https://doi.org/10.1007/s00726-007-0583-3

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  • DOI: https://doi.org/10.1007/s00726-007-0583-3

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