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Amino Acids

, Volume 35, Issue 2, pp 275–281 | Cite as

Experimental study on vasoactive intestinal peptide (VIP) and its diaminopropane bound (VIP-DAP) analog in solution

  • M. Caraglia
  • M. Carteni
  • A. Dicitore
  • D. Cassese
  • S. De Maria
  • P. Ferranti
  • G. Giuberti
  • A. Abbruzzese
  • P. Stiuso
Review Article

Summary.

Bioactive peptides represent an exciting area of research in the fields of biochemistry and medicine and in particular the VIP/PACAP network appears to be of interest. Vasoactive intestinal peptide (VIP) is a pleiotropic factor that exerts a physiological regulatory influence and is involved in the pathogenesis of several human disorders. In this paper we have reported structural characterization of VIP by experimental and computational methods as well as a comparative analysis of the peptide with its transglutaminase catalyzed analog VIP-Diaminopropane (VIP-DAP).

Keywords: Vasoactive intestinal peptide (VIP) – Transglutaminase (TGase) – Limited proteolysis – Computational study – VPAC receptors 

Abbreviations:

cAMP

cyclic AMP

CBP

CREB binding protein

CRE

cAMP responsive element

CREB

CRE-binding protein

CyRE

cytokine response element

GAS

gamma activated sequences

IFN-γ

interferon gamma

IL-6

interleukin 6

iNOS

inducible nitric oxide synthase

IRF-1

interferon regulatory factor 1

LPS

lipopolysaccharide

PACAP

Pituary adenylate cyclase-activating polypeptide

PKA

protein kinase A

TNF-α

tumor necrosis factor α

VIP

vasoactive intestinal peptide

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • M. Caraglia
    • 1
  • M. Carteni
    • 2
  • A. Dicitore
    • 3
  • D. Cassese
    • 3
  • S. De Maria
    • 2
  • P. Ferranti
    • 4
  • G. Giuberti
    • 3
  • A. Abbruzzese
    • 3
  • P. Stiuso
    • 3
  1. 1.Dipartimento di Oncologia SperimentaleIstituto Nazionale Tumori “Fondazione G. Pascale”NaplesItaly
  2. 2.Dipartimento di Medicina SperimentaleSeconda Università degli Studi di NapoliNapoliItaly
  3. 3.Dipartimento di Biochimica e BiofisicaSeconda Università degli Studi di NapoliNapoliItaly
  4. 4.Dipartimento di Scienza degli AlimentiUniversità di Napoli “Federico II”, Parco GussonePorticiItaly

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