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In silico analysis of arginine catabolism as a source of nitric oxide or polyamines in endothelial cells

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Summary.

We use a modeling and simulation approach to carry out an in silico analysis of the metabolic pathways involving arginine as a precursor of nitric oxide or polyamines in aorta endothelial cells. Our model predicts conditions of physiological steady state, as well as the response of the system to changes in the control parameter, external arginine concentration. Metabolic flux control analysis allowed us to predict the values of flux control coefficients for all the transporters and enzymes included in the model. This analysis fulfills the flux control coefficient summation theorem and shows that both the low affinity transporter and arginase share the control of the fluxes through these metabolic pathways.

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Authors and Affiliations

  1. Procel Group, Department of Molecular Biology and Biochemistry, University of Málaga, and CIBERER, Málaga, Spain

    R. Montañez, C. Rodríguez-Caso, F. Sánchez-Jiménez & M. Á. Medina

Authors
  1. R. Montañez
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  2. C. Rodríguez-Caso
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  3. F. Sánchez-Jiménez
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  4. M. Á. Medina
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Additional information

The first two authors equally contributed to this work.

Current address for the second author: ICREA-Complex Systems Laboratory, Universitat Pompeu Fabra, Barcelona, Spain.

Authors’ address: Dr. Miguel Ángel Medina, Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Universidad de Málaga, E-29071 Málaga, Spain

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Montañez, R., Rodríguez-Caso, C., Sánchez-Jiménez, F. et al. In silico analysis of arginine catabolism as a source of nitric oxide or polyamines in endothelial cells. Amino Acids 34, 223–229 (2008). https://doi.org/10.1007/s00726-007-0502-7

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  • DOI: https://doi.org/10.1007/s00726-007-0502-7

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