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A new selective pre-column ninhydrin-based derivatization for a RP-HPLC determination of plasma asymmetric dimethyl-l-arginine (ADMA) by fluorescence detection

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Abstract

We report a new selective and direct pre-column ninhydrin-based derivatization reaction for determination of plasma ADMA levels. This original derivatization procedure matched to a validated and rapid RP-HPLC method can be a useful alternative to other assays in which time consuming and expensive extraction and/or purification steps are required.

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Authors and Affiliations

  1. Department of Biomedical Sciences, Chair of Clinical Biochemistry, University of Sassari, Viale San Pietro 43/B, 07100, Sassari, Italy

    Salvatore Sotgia, Angelo Zinellu, Luca Deiana & Ciriaco Carru

  2. Department of Medicinal and Toxicological Chemistry, University of Sassari, Sassari, Italy

    Salvatore Sotgia & Gerard Aime Pinna

  3. National Laboratory of the National Institute of Biostructures and Biosystems, Osilo, Italy

    Ciriaco Carru

Authors
  1. Salvatore Sotgia
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  2. Angelo Zinellu
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  3. Gerard Aime Pinna
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  4. Luca Deiana
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  5. Ciriaco Carru
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Correspondence to Salvatore Sotgia or Ciriaco Carru.

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Sotgia, S., Zinellu, A., Pinna, G.A. et al. A new selective pre-column ninhydrin-based derivatization for a RP-HPLC determination of plasma asymmetric dimethyl-l-arginine (ADMA) by fluorescence detection. Amino Acids 34, 677–682 (2008). https://doi.org/10.1007/s00726-007-0001-x

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  • DOI: https://doi.org/10.1007/s00726-007-0001-x

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