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Cadmium and heat response of the fungus Heliscus lugdunensis isolated from highly polluted and unpolluted areas

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Summary.

Induction of heat shock protein (Hsp) 70 and distinct metallothionein-like proteins (MTLPs) in response to Cd and heat treatment were studied in two strains of the aquatic hyphomycete Heliscus lugdunensis: Hl-H4, isolated from a heavy metal polluted site, and Hl-BB taken from an unpolluted area. Upon Cd-exposure, Hsp70 was actively synthesized in the strain Hl-H4, and to a much lower degree in the strain Hl-BB. The Hsp70-expression was time- and dose-dependent, reaching a maximum after 24 h incubation with 80 µM Cd. Upon heat-stress, a similar response was observed: a strong Hsp70-expression in Hl-H4, and only a marginal one in Hl-BB. The strains reacted to Cd-exposure by a specific, environmentally related induction of MTLPs, as shown by the highly sensitive bimane derivatisation method of SH-rich proteins. In Hl-H4, a strong expression of 11 kDa MTLP was registered, which followed strictly the induction pattern of Hsp70. This suggests interdependence of the induction mechanisms and roles of these stress proteins in metal resistance. On the contrary, in Hl-BB a weak expression of MTLP of about 20 kDa was observed, exhibiting completely different induction pattern. The results suggest that the specific induction of Hsp70 and/or distinct MTLPs in the range of 11 kDa in H. lugdunensis strain Hl-H4 are essential adaptive mechanisms to continuous heavy metal exposure.

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Abbreviations

AA:

acrylamide

AP:

alkaline phosphatase

APS:

ammonium peroxydisulphate

Bis-N:

N′-methylene-bis-acrylamide

BCIP:

5-bromo-4-chloro-e-indolyl phosphate disodium salt

DTE:

dithiothreitol

DTPA:

bis-(2-aminoethyl)-amino-N,N,N′N″N″-pentaacetic acid

ECL:

electrogenerated chemoluminescence

EDTA:

ethylenediamine tetraacetic acid

NC:

nitrocellulose

PMSF:

phenylmethylsulphonylfluoride

PAGE:

polyacrylamide gel electrophoresis

SDS:

sodium dodecyl sulphate

PBS:

phosphate buffered saline

Hsp:

heat shock protein

MT:

metallothionein

MTLP:

metallothionein-like protein

NBT:

p-nitro blue tetrazolium chloride

TCA:

trichloroacetic acid

TCEP:

tris-(2-carboxyethyl) phosphine chloride

Tris:

tris(hydroxymethyl)aminomethane

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Authors and Affiliations

  1. Institute of Biochemistry, Martin-Luther-University, Halle, Germany

    J. Miersch

  2. Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia, Bulgaria

    K. Grancharov

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  1. J. Miersch
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  2. K. Grancharov
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Authors’ address: Assoc. Prof. Dr. Konstantin Grancharov, Bulgarian Academy of Sciences, Institute of Molecular Biology, Acad. G. Bonchev Str. Bl. 21, BG-1113 Sofia, Bulgaria

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Miersch, J., Grancharov, K. Cadmium and heat response of the fungus Heliscus lugdunensis isolated from highly polluted and unpolluted areas. Amino Acids 34, 271–277 (2008). https://doi.org/10.1007/s00726-006-0491-y

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  • DOI: https://doi.org/10.1007/s00726-006-0491-y

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