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Applications and current challenges of proteomic approaches, focusing on two-dimensional electrophoresis

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Summary.

Since the formulation of the concept of “proteomics” in 1995, a plethora of proteomic technologies have been developed in order to study proteomes of tissues, cells and organelles. The powerful new technologies enabled by proteomic approaches have lead to the application of these methods to an exponentially increasing variety of biological questions for highly complex protein mixtures. Continuous technical optimization allows for an ever-increasing sensitivity of proteomic techniques. In this review, a brief overview of currently available proteomic techniques and their applications is given, followed by a more detailed description of advantages and technical challenges of two-dimensional electrophoresis (2-DE). Some solutions to circumvent currently encountered technical difficulties for 2-DE analyses are proposed.

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Abbreviations

ASB-14:

Amidosulfobetaine-14

CHAPS:

3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate

2-DE:

two-dimensional electrophoresis

DIGE:

difference gel electrophoresis

HUPO:

human proteome organization

IEF:

isoelectric focusing

SDS-PAGE:

sodium dodecyl sulfate polyacrylamide gel electrophoresis

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Authors and Affiliations

  1. Douglas Hospital Research Center, Department of Psychiatry, McGill University, Montréal, Québec, Canada

    F. G. G. Vercauteren & R. Quirion

  2. Laboratory of Neuroplasticity and Neuroproteomics, Katholieke Universiteit Leuven, Leuven, Belgium

    F. G. G. Vercauteren & L. Arckens

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  1. F. G. G. Vercauteren
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  2. L. Arckens
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  3. R. Quirion
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Vercauteren, F., Arckens, L. & Quirion, R. Applications and current challenges of proteomic approaches, focusing on two-dimensional electrophoresis. Amino Acids 33, 405–414 (2007). https://doi.org/10.1007/s00726-006-0460-5

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