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Protein-carbonyl accumulation in the non-replicative senescence of the methionine sulfoxide reductase A (msrA) knockout yeast strain

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Summary.

The major enzyme of the methionine sulfoxide reductase (Msr) system is MsrA. Senescing msrA knockout mother yeast cells accumulated significant amounts of protein-carbonyl both at 5 generation-old (young) and 21 generation-old (old) cultures, while the control mother cells showed significant levels of protein-carbonyl mainly in the old culture. The Msr activities of both yeast strains declined with age and exposure of cells to H2O2 caused an accumulation of protein-carbonyl especially in the msrA knockout strain. It is suggested that a compromised MsrA activity may serve as a marker for non-replicative aging.

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Authors and Affiliations

  1. Department of Pharmacology and Toxicology, School of Pharmacy, University of Kansas, Lawrence, KS, U.S.A.

    D. Oien & J. Moskovitz

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  1. D. Oien
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  2. J. Moskovitz
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Oien, D., Moskovitz, J. Protein-carbonyl accumulation in the non-replicative senescence of the methionine sulfoxide reductase A (msrA) knockout yeast strain. Amino Acids 32, 603–606 (2007). https://doi.org/10.1007/s00726-006-0448-1

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  • DOI: https://doi.org/10.1007/s00726-006-0448-1

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